PBFI AM (Synonyms: Potassium-binding Benzofuran Isophthalate Acetoxymethyl ester) |
Catalog No.GC18477 |
PBFI AM is a cell-permeable, potassium-sensitive fluorophore used to measure potassium changes in cells and intracellular compartments.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 124549-23-1
Sample solution is provided at 25 µL, 10mM.
Quality Control & SDS
- View current batch:
- Purity: >95.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
PBFI AM is a cell-permeable, potassium-sensitive fluorophore used to measure potassium changes in cells and intracellular compartments.[1] [2] As PBFI AM enters cells, it is hydrolyzed by intracellular esterases to produce PBFI. The affinity of PBFI for potassium is sodium-dependent (Kd = 44 and 5.1 mM with or without sodium, respectively). [3] Absorption and emission maxima are 336 and 480-557 nm, respectively.[4] [5]
Reference:
[1]. Grieshaber, S., Swanson, J.A., and Hackstadt, T. Determination of the physical environment within the Chlamydia trachomatis inclusion using ion-selective ratiometric probes. Cell. Microbiol. 4(5), 273-283 (2002).
[2]. Ljubkovic, M., Marinovic, J., Fuchs, A., et al. Targeted expression of Kir6.2 in mitochondria confers protection against hypoxic stress. J. Physiol. 577(Pt 1), 17-29 (2006).
[3]. Halperin, S.J., and Lynch, J.P. Effects of salinity on cytosolic Na+ and K+ in root hairs of Arabidopsis thaliana: In vivo measurements using the fluorescent dyes SBFI and PBFI. J. Exp. Bot. 54(390), 2035-2043 (2003).
[4]. Sabnis, R.W. Handbook of biological dyes and stains: Synthesis and industrial applications. (2010).
[5]. Jezek, P., Mahdi, F., and Garlid, K.D. Reconstitution of the beef heart and rat liver mitochondrial K+/H+ (Na+/H+) antiporter. Quantitation of K+ transport with the novel fluorescent probe, PBFI. J. Biol. Chem. 265(18), 10522-10526 (1990).
Cas No. | 124549-23-1 | SDF | |
Synonyms | Potassium-binding Benzofuran Isophthalate Acetoxymethyl ester | ||
Chemical Name | 4,4'-[1,4,10,13-tetraoxa-7,16-diazacyclooctadecane-7,16-diylbis(5-methoxy-6,2-benzofurandiyl)]bis-1,3-benzenedicarboxylic acid, tetrakis[(acetyloxy)methyl] ester | ||
Canonical SMILES | COC1=CC2=C(OC(C3=C(C(OCOC(C)=O)=O)C=C(C(OCOC(C)=O)=O)C=C3)=C2)C=C1N4CCOCCOCCN(C5=CC(OC(C6=C(C(OCOC(C)=O)=O)C=C(C(OCOC(C)=O)=O)C=C6)=C7)=C7C=C5OC)CCOCCOCC4 | ||
Formula | C58H62N2O24 | M.Wt | 1171.1 |
Solubility | Soluble in methanol and DMSO | Storage | Store at -20°C |
General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. |
Prepare stock solution | |||
1 mg | 5 mg | 10 mg | |
1 mM | 0.8539 mL | 4.2695 mL | 8.539 mL |
5 mM | 0.1708 mL | 0.8539 mL | 1.7078 mL |
10 mM | 0.0854 mL | 0.4269 mL | 0.8539 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Average Rating: 5
(Based on Reviews and 27 reference(s) in Google Scholar.)GLPBIO products are for RESEARCH USE ONLY. Please make sure your review or question is research based.
Required fields are marked with *