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Phenol Red sodium salt (Phenolsulfonephthalein sodium salt)

Catalog No.GC30235

Phenol red (Phenolsulfonephthalein) sodium salt is a pH indicator dye, relying on a distinct color change from pink to yellow in case of a positive reaction .

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Phenol Red sodium salt (Phenolsulfonephthalein sodium salt) Chemical Structure

Cas No.: 34487-61-1

Size Price Stock Qty
5g
$50.00
In stock
10mM (in 1mL Water)
$56.00
In stock
25g
$101.00
In stock

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Sample solution is provided at 25 µL, 10mM.

Product Documents

Quality Control & SDS

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Protocol

Cell experiment:

Phenol red is dissolved in 0.1% ethanol. To determine the effect of phenol red on cell proliferation, MCF-7 cells grown for 1 week before experiments in phenol red-free MEM supplemented are harvested and seeded into T-25 flasks (ca. 1.5 x 105 cells per flask). The following day, cells from three flasks are harvested and counted with a Coulter Counter. Then the medium is changed to phenol red- and insulin-free MEM, which contains various concentrations of dextran-coated charcoal-treated calf serum, phenol red (0-300 μM), tamoxifen, hydroxytamoxifen, estradiol, or ethanol vehicle (0.1%), and cell number is monitored as a function of time[1]. Phenol Red sodium salt is dissolved in distilled water as a 10 g/L stock solution[2].

References:

[1]. Berthois Y, et al. Phenol red in tissue culture media is a weak estrogen: implications concerning the study of estrogen-responsive cells in culture. Proc Natl Acad Sci U S A. 1986 Apr;83(8):2496-500.
[2]. Pick E, et al. A simple colorimetric method for the measurement of hydrogen peroxide produced by cells in culture. J Immunol Methods. 1980;38(1-2):161-70.

Background

Phenol Red sodium salt is a water soluble pH indicator used in the 6.8 (yellow) to 8.2 (red) range.

Phenol Red sodium salt is commonly employed as a cell tissue culture pH marker. Under normal conditions, phenol red added to the growth medium will have a pink-red color. Dying cells or overgrowth of contaminants will cause a change in pH resulting in a change in indicator color. Phenol red bears a structural resemblance to some nonsteroidal estrogens and has significant estrogenic activity at the concentrations (15-45 μM) at which it is found in tissue culture media. Phenol red binds to the estrogen receptor of MCF-7 human breast cancer cells and stimulates the proliferation of estrogen receptor-positive MCF-7 breast cancer cells in a dose-dependent manner but has no effect on the growth of estrogen receptor-negative MDA-MB-231 breast cancer cells[1]. Phenol red is a simple, rapid and inexpensive method for the measurement of hydrogen peroxide produced by cells in culture. The assay is based on the horseradish peroxidase-mediated oxidation of phenol red by hydrogen peroxide which results in the formation of a compound demonstrating increased absorbance at 610 nm[2].

[1]. Berthois Y, et al. Phenol red in tissue culture media is a weak estrogen: implications concerning the study of estrogen-responsive cells in culture. Proc Natl Acad Sci U S A. 1986 Apr;83(8):2496-500. [2]. Pick E, et al. A simple colorimetric method for the measurement of hydrogen peroxide produced by cells in culture. J Immunol Methods. 1980;38(1-2):161-70.

Chemical Properties

Cas No. 34487-61-1 SDF
Canonical SMILES O=S1(OC(C2=CC=C([O-])C=C2)(C3=CC=C(O)C=C3)C4=CC=CC=C41)=O.[Na+]
Formula C19H13NaO5S M.Wt 376.36
Solubility Water : ≥ 100 mg/mL (265.70 mM) Storage Store at 4°C, protect from light
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table

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1 mg 5 mg 10 mg
1 mM 2.657 mL 13.2852 mL 26.5703 mL
5 mM 0.5314 mL 2.657 mL 5.3141 mL
10 mM 0.2657 mL 1.3285 mL 2.657 mL
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.

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Average Rating: 5 ★★★★★ (Based on Reviews and 16 reference(s) in Google Scholar.)

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