PKA Inhibitor (5-24) (trifluoroacetate salt) (Synonyms: PKI (524), Protein Kinase A Inhibitor (524))

Name: PKA Inhibitor (5-24) (trifluoroacetate salt)
Brand: GlpBio
SKU: GC48329
Availability: InStock
Rating: 5 (13 reviews)

Catalog No.GC48329

A synthetic peptide inhibitor of PKA

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PKA Inhibitor (5-24) (trifluoroacetate salt) Chemical Structure

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500µg	$101.00	In stock
1mg	$171.00	In stock
5mg	$709.00	In stock

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Sample solution is provided at 25 µL, 10mM.

GlpBio Products Cited In Reputable Papers

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610.7931 (2022): 366-372

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Description Chemical Properties Product Documents

Product Documents

Quality Control & SDS

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Purity: >95.00%

PKI PKA Inhibitor (5-24) is a synthetic peptide inhibitor of PKA (cAMP-dependent protein kinase) (K_i = 2.3 nM) derived from the active site of the skeletal muscle inhibitor protein.¹ It mimics the protein substrate by binding to the catalytic site through the arginine-cluster basic subsite.¹ The prominent enzyme-substrate interaction site occurs where PKA catalytic subunit residues Tyr²³⁵ and Phe²³⁹ form a sandwich-like structure with residue Phe¹⁰ of PKI (5-24).²

1.Cheng, H.C., Kemp, B.E., Pearson, R.B., et al.A potent synthetic peptide inhibitor of the cAMP-dependent protein kinaseThe Journal of Biological Chemisty261(3)989-992(1986) 2.Bossemeyer, D., Engh, R.A., Kinzel, V., et al.Phosphotransferase and substrate binding mechanism of the cAMP-dependent protein kinase catalytic subunit from porcine heart as deduced from the 2.0 Å structure of the complex with Mn2+ adenylyl imidodiphosphate and inhibitor peptide PKI(5-24)EMBO Journal12(3)849-859(1993)

Cas No.		SDF
Synonyms	PKI (524), Protein Kinase A Inhibitor (524)
Canonical SMILES	[H]N[C@]([C@@H](C)O)([H])C(N[C@]([C@@H](C)O)([H])C(N[C@H](C(N[C@H](C(N[C@H](C(N[C@H](C(N[C@]([C@H](CC)C)([H])C(N[C@H](C(N[C@@H](CO)C(NCC(N[C@@H](CCCNC(N)=N)C(N[C@]([C@@H](C)O)([H])C(NCC(N[C@@H](CCCNC(N)=N)C(N[C@@H](CCCNC(N)=N)C(N[C@@H](CC(N)=O)C(N[C@H](C(N[C@]([C@H](CC)C)([H])C(N[C@H](C(N[C@H](C(O)=O)CC(O)=O)=O)CC1=CN=CN1)=O)=O)C)=O)=O)=O)=O)=O)=O)=O)=O)=O)C)=O)=O)CC2=CC=CC=C2)=O)CC(O)=O)=O)C)=O)CC3=CC=C(O)C=C3)=O)=O.OC(C(F)(F)F)=O
Formula	C94H148N32O31•XCF3COOH	M.Wt	2222.4
Solubility	DMSO: 1 mg/ml,PBS (pH 7.2): 2 mg/ml	Storage	-20°C
General tips	Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition	Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table

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		1 mg	5 mg	10 mg
	1 mM	0.45 mL	2.2498 mL	4.4996 mL
	5 mM	0.09 mL	0.45 mL	0.8999 mL
	10 mM	0.045 mL	0.225 mL	0.45 mL

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
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3. All of the above co-solvents are available for purchase on the GlpBio website.

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Average Rating: 5 ★★★★★ (Based on Reviews and 13 reference(s) in Google Scholar.)

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PKA Inhibitor (5-24) (trifluoroacetate salt) (Synonyms: PKI (524), Protein Kinase A Inhibitor (524))

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