Protease and Phosphatase Inhibitor Cocktail (EDTA Free, 100X in ddH2O) |
| Catalog No.GK10015 |
Protease and phosphatase inhibitor cocktail (EDTA-free,100X in ddH2O) is suitable for cell lysis and protein extraction of mammalian cells, primary cells, yeast cells, bacteria, animal tissues and plant tissues to maintain the stability and phosphorylation status of proteins in the sample.
Products are for research use only. Not for human use. We do not sell to patients.
Sample solution is provided at 25 µL, 10mM.
This product is a ready-to-use mixture of protease and phosphatase inhibitors (EDTA-free, 100X in ddH2O), which can effectively inhibit the activity of proteases and phosphatases in conventional cell lysis and protein extraction. This product is suitable for experiments such as protein extraction from mammalian cells, primary cells, yeast cells, bacteria, animal tissues and plant tissues.
The main ingredients of this product include: serine|cysteine protease inhibitor Leupeptin, bovine trypsin inhibitor Aprotinin, aminopeptidase inhibitor Bestatin, cysteine protease inhibitor E-64, serine|threonine phosphatase reversible inhibitor β-glycerophosphate, serine|threonine phosphatase irreversible inhibitor Sodium Pyrophosphate, acid phosphatase inhibitor Sodium Fluoride, alkaline phosphatase|tyrosine phosphatase inhibitor Sodium Orthovanadate. In addition, this product does not contain EDTA, and metalloproteinases and aspartic acid proteases are not inhibited.
This product can effectively inhibit the functions of proteases such as serine proteases, leucine aminopeptidase, cysteine protease and aminopeptidase B, as well as phosphatases such as serine|threonine phosphatases, protein tyrosine phosphatases, acid phosphatases and alkaline phosphatases.
Recommended usage: When lysing cells or extracting proteins, mix this product with lysis buffer at a volume ratio of 1:100.
Thaw at room temperature, add at 1:100 (v/v) dilution to solution samples (such as cell lysates or tissue extracts), before assaying.
Application Fields: Western Blot analysis, Co-IP, pull-down, IF, IHC, kinase assay and etc.
| Product Name | Summary | CAS Number | Targets |
| Leupeptin | Serine and cysteine protease inhibitors | 103476-89-7 | Proteases | Serine Protease |
| Aprotinin | Bovine trypsin inhibitors | 9087-70-1 | Proteases | Serine Protease |
| E-64 | Cysteine protease inhibitors | 66701-25-5 | Proteases | Cathepsin |
| Bestatin hydrochloride | Aminopeptidase inhibitors | 65391-42-6 | Proteases | Aminopeptidase |
| Sodium Orthovanadate | PTP inhibitors | 13721-39-6 | Phosphatase | Tyrosine Phosphatases |
| Sodium fluoride | Acid phosphatase inhibitors | 7681-49-4 | Phosphatase | Serine and Threonine Phosphatases |
| β-glycerophosphate | Reversible inhibitors of serine/threonine phosphatase | 13408-09-8 | Phosphatase | Serine and Threonine Phosphatases |
| Sodium pyrophosphate | Irreversible inhibitor of serine/threonine phosphatase | 7722-88-5 | Phosphatase | Serine and Threonine Phosphatases |
| Store at -20°C for 1 year. | |||
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)
GLPBIO products are for RESEARCH USE ONLY. Please make sure your review or question is research based.
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