Protease Inhibitor Cocktail (100X in DMSO, EDTA plus) |
| Catalog No.GK10022 |
Inhibit proteolytic degradation during cell lysis and protein extraction.
Products are for research use only. Not for human use. We do not sell to patients.
Sample solution is provided at 25 µL, 10mM.
Protease Inhibitor Cocktail (100X in DMSO, EDTA plus), which is used during cell lysis and protein extraction, is a ready-to-use concentrated stock solutions of multiple protease inhibitors for endogenous protease. This product contains a separate 0.5M EDTA solution which inhibiting metalloproteases.
It generates optimized concentrations of six broad-spectrum protease inhibitors stabilized in DMSO, that powerfully inhibits serine-proteases, cysteine-proteases, aspartic acid-proteases and aminopeptidases appearing in cellular lysate samples. EDTA provides inhibition for metalloproteases.
EDTA inhibits metalloproteases by chelating the divalent cations that is necessary for their activity, so the activities of other proteins may be affected by EDTA. Therefore, empirical testing may be need in particular experiments to determine if EDTA will take a bad impact. If the protein of interest is to be purified using immobilized metal chelate affinity chromatography (IMAC) or analyzed by 2D gel electrophoresis, EDTA must be removed by extensive dialysis or desalting before purifying.
| Tube | Ingredient | M.W. | Target |
| A (100X in DMSO) | |||
| AEBSF | 239.5 | Serine proteases | |
| Aprotinin | 6511.5 | Serine proteases | |
| Bestatin | 308.38 | Aminopeptidases | |
| E-64 | 357.4 | Cysteine proteases | |
| Leupeptin | 475.6 | Alkaline phosphatases | |
| Pepstatin A | 685.9 | Aspartic proteases | |
| B (100X in ddH2O) | EDTA | 372.24 | Protease |
Thaw on ice, add at 1:100 (v/v) dilution to solution samples (such as cell lysates or tissue extracts) before assaying.
Applications: WB, Co-IP, pull-down, IF, IHC, Flow Cytometry, kinase assay and etc.
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Average Rating: 5 (Based on Reviews and 19 reference(s) in Google Scholar.)
GLPBIO products are for RESEARCH USE ONLY. Please make sure your review or question is research based.
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