Protease Inhibitor Cocktail II (EDTA-Free, 200X in DMSO) |
| Catalog No.GK10016 |
Equivalent to Sigma P1860, for use in tissue culture media to prevent proteolytic degradation of secreted proteins.
Products are for research use only. Not for human use. We do not sell to patients.
Sample solution is provided at 25 µL, 10mM.
Secreted protein production is halted and degradation is increased when proteins are extracted from media in vitro. Crude cell extracts contain a number of endogenous enzymes, such as phosphatases and proteases, which are capable of degrading proteins in the extracts. The best way to increase the yield of intact proteins is to add inhibitors of those enzymes known to be present.
Protease inhibitor cocktail is used in tissue culture media to increase protein stability. The cocktail functions to inhibit proteases that would degrade either non-phosphorylated or phosphorylated protein substrates. This cocktail should be used as a supplement to tissue culture media to prevent the degradation of secreted proteins.
This protease inhibitor cocktail contains individual components, including Aprotinin, Bestatin, E-64, Leupeptin and Pepstatin A with a broad specificity for cysteine, serine, aspartic and aminopeptidases. This protease inhibitor cocktail has been optimized and tested for tissue culture media. This protease inhibitor cocktail is supplied as a ready-to-use solution in DMSO.
| Ingredient | Summary | CAS Number | Targets |
| Aprotinin | Inhibitor of bovine pancreatic trypsin | 9087-70-1 | Proteases|Serine Protease |
| Bestatin | Aminopeptidase inhibitor | 58970-76-6 | Proteases|Aminopeptidase |
| E-64 | Cysteine protease inhibitor,irriversible | 66701-25-5 | Proteases|Cathepsin |
| Leupeptin, Microbial | Inhibitor of serine and cysteine proteases | 103476-89-7 | Proteases|Serine Protease |
| Pepstatin A | Aspartic proteinases inhibitor | 26305-03-3 | Proteases|Other Proteases |
It is suggested to dilute the cocktail appropriately for specific cell lines. The test should begin with at least 200-fold dilution because concentrations of DMSO greater than 0.5% may be deleterious for cells. Since various cell lines will differ in their sensitivity, further dilutions may be needed for this cocktail. This cocktail remains effective for up to 48 hours in the medium. After 48 hours, the medium should be replaced with fresh medium containing the cocktail.
Applications: WB, Co-IP, pull-down, IF, IHC, kinase assay and etc
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