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Protease Inhibitor Cocktail III (EDTA-Free, 100X in DMSO)

Catalog No.GK10017

Equivalent to Sigma P8215, for use with fungal and yeast extracts to increase protein stability.

Products are for research use only. Not for human use. We do not sell to patients.

Protease Inhibitor Cocktail III (EDTA-Free, 100X in DMSO) Chemical Structure

Size Price Stock Qty
1mL
$18.40
In stock
1mL x 10
$130.00
In stock
1mL x 50
$520.00
In stock
1mL x 100
$780.00
In stock

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Sample solution is provided at 25 µL, 10mM.

Product has been cited by 2 publications

Product Documents

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Protocol

Thaw at room temperature, add at 1:100 (v/v) dilution to solution samples (such as yeast extracts) before assaying.

Applications: WB, Co-IP, pull-down, IF, IHC, kinase assay and etc.

Background

Endogenous proteins are produced and degraded in a balanced state, so their cellular levels are stable under stable environmental conditions. Crude cell extracts contain a number of endogenous enzymes, such as phosphatases and proteases, which are capable of degrading proteins in the extracts. Protein production is greatly halted and degradation is increased when proteins are extracted from cells and tissues in vitro. The best way to increase the yield of intact proteins is to add inhibitors of those enzymes known to be present.

Protease inhibitor cocktail is used with fungal and yeast extracts to increase protein stability. The cocktail functions to inhibit proteases that would degrade either non-phosphorylated or phosphorylated protein substrates.

his protease inhibitor cocktail contains individual components, including AEBSF, 1,10-Phenanthroline, E-64 and Pepstatin A with a broad specificity for cysteine, serine, aspartic, and metalloproteases. This cocktail has been optimized and tested for fungal and yeast cell use. This protease inhibitor cocktail is supplied as a ready-to-use solution in DMSO.

Ingredient Summary CAS Number Targets
o-Phenanthroline metalloprotease inhibitor 66-71-7 Proteases|Other Proteases
AEBSF.HCl Serine protease inhibitor 30827-99-7 Proteases|Serine Protease
E-64 Cysteine protease inhibitor,irriversible 26305-03-3 Proteases|Cathepsin
Pepstatin A Aspartic proteinases inhibitor 103476-89-7 Proteases|Other Proteases

Reviews

Review for Protease Inhibitor Cocktail III (EDTA-Free, 100X in DMSO)

Average Rating: 5 ★★★★★ (Based on Reviews and 16 reference(s) in Google Scholar.)

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Review for Protease Inhibitor Cocktail III (EDTA-Free, 100X in DMSO)

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