Miquelianin (Quercetin 3-O-glucuronide) (Synonyms: Miquelianin, Quercetin 3-O-β-Dglucuronide) |
| Catalog No.GC44795 |
Miquelianin (Quercetin 3-O-glucuronide) is the main metabolite of quercetin in the body and is also a carbonyl reductase 1 (CBR1) inhibitor.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 22688-79-5
Sample solution is provided at 25 µL, 10mM.
_1-3.$$$39978408@51.jpg');)
_1-9.$$$38538795@65.jpg');)
_1-9.$$$39112701@51.jpg');)
_1-7.$$$37316672@70.jpg');)
_366-372.$$$36198801@70.jpg');)
.$$$38565142@65.jpg');)
_1867-1883.$$$33248023@67.jpg');)
_eads6055.$$$39977546@45.jpg');)
_eadm9805.$$$40080571@45.jpg');)
_eadj3020.$$$39666821@45.jpg');)
_1-20.$$$39757301@28.jpg');)
_1-24.$$$38898113@28.jpg');)
_273-287.$$$36806353@46.jpg');)
_1-16.$$$37156877@44.jpg');)
_1-19.$$$37460805@44.jpg');)
_1291-1307.$$$34518654@46.jpg');)
Miquelianin (Quercetin 3-O-glucuronide) is the main metabolite of quercetin in the body and is also a carbonyl reductase 1 (CBR1) inhibitor [1]. Miquelianin effectively scavenges reactive oxygen species (ROS), inhibits inflammatory responses mediated by the NF-κB signaling pathway, and improves cellular metabolic homeostasis by activating the PI3K/Akt pathway [2]. Miquelianin is primarily used in experimental studies of oxidative, anti-inflammatory, neuroprotective, and metabolic regulatory mechanisms [3-4].
In MDCK cells, Miquelianin can effectively inhibit the replication of H1N1-UI182 virus, as manifested by a significant decrease in the expression of viral nucleoprotein (NP) and a significant reduction in cytopathic effect (CPE) observed under a microscope [5]. In C3H10T1/2 cells, Miquelianin (5μM, 10μM; 24h) can reduce intracellular lipid accumulation by promoting lipolysis and fatty acid β-oxidation and inhibiting lipid droplet formation, thereby exerting metabolic regulation and anti-fatty degeneration effects [6].
In atopic dermatitis (AD) mice model, Oral administration of Miquelianin (4mg/mL, 10mg/mL; po; 21d) can significantly reduce serum IgE levels and the expression of Th2-related cytokines (such as IL-4 and IL-5) in the spleen, thereby inhibiting excessive Th2 immune responses [7]. In HFD-fed mice model, Miquelianin (12.8mg/kg, 25.6mg/kg; ig; 12weeks) extract promotes white-to-beige fat conversion via blocking AMPK/DRP1/mitophagy and modulating gut microbiota [8].
References:
[1]. Terao J, Yamaguchi S, Shirai M, et al. Protection by quercetin and quercetin 3-O-β-D-glucuronide of peroxynitrite-induced antioxidant consumption in human plasma low-density lipoprotein[J]. Free Radical Research, 2001, 35(6): 925-931.
[2]. Wang T, Lv L, Feng H, et al. Unlocking the potential: quercetin and its natural derivatives as promising therapeutics for sepsis[J]. Biomedicines, 2024, 12(2): 444.
[3]. Bakirhan N K, Sener S O, Baran M Y, et al. An Electrochemical Approach for a Flavonoid: Miquelianin via Carbon-Based Electrodes and Its Analysis from Calystegia silvatica[J]. Journal of AOAC International, 2025: qsaf032.
[4]. Schepetkin I A, Ramstead A G, Kirpotina L N, et al. Therapeutic potential of polyphenols from Epilobium angustifolium (Fireweed)[J]. Phytotherapy research, 2016, 30(8): 1287-1297.
[5]. Li H, Shen B, Bi Y, et al. Miquelianin inhibits IAV infection via the MAPK signaling pathway both in vitro and in vivo[J]. Frontiers in Immunology, 2025, 16: 1532336.
[6]. Wang Z, Xue C, Yang T, et al. Miquelianin, a main functional flavonoid of lotus leaf, induces thermogenic signature via p38‐PINK1‐PARKIN‐mediated mitophagy and mimicking NRF2 signaling during brown adipocyte differentiation[J]. Food Frontiers, 2023, 4(4): 1831-1844.
[7]. Choi D W, Jung S Y, Kim G D, et al. Miquelianin inhibits allergic responses in mice by suppressing CD4+ T cell proliferation[J]. Antioxidants, 2021, 10(7): 1120.
[8]. Wang Z, Yang T, Zeng M, et al. Miquelianin in Folium Nelumbinis extract promotes white-to-beige fat conversion via blocking AMPK/DRP1/mitophagy and modulating gut microbiota in HFD-fed mice[J]. Food and Chemical Toxicology, 2023, 181: 114089.
| Cell experiment [1]: | |
Cell lines | MDCK cells |
Preparation Method | MDCK cells were cultured in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% FBS, 100IU/mL penicillin, and 100μg/mL streptomycin at 37℃ and 5% CO2. When the cell density reached 70–80%, the supernatant was discarded, and different concentrations of the Miquelianin (6.25, 12.5, 25, 50, and 100μM) were plated in duplicate in five replicate wells. Virus-infected and uninfected cells were simultaneously treated with the working solution at different concentrations. Positive control wells (H1N1-UI182 virus only) and negative control wells (no H1N1-UI182 virus) were assigned to each plate. After 48 hours, cell viability was assessed using a CCK-8 assay. |
Reaction Conditions | 6.25-100μM; 48h |
Applications | Miquiline can effectively inhibit the replication of H1N1-UI182 virus, as manifested by a significant decrease in the expression of viral nucleoprotein (NP) and a significant reduction in cytopathic effect (CPE) observed under a microscope. |
| Animal experiment [2]: | |
Animal models | Atopic dermatitis (AD) mice model |
Preparation Method | To evaluate the anti-AD effects of oral Miquelianin treatment, the BALB/c mice were divided into five groups: naïve (TMA-non-exposed, n = 6), sham (untreated, n = 8), Miquelianin-4 (4mg/mL, n = 8), Miquelianin-10 (10mg/mL, n = 8), and Dex (1mg/mL, n = 6). Mice were sensitized on the shaved right flank with 50μL of 5% TMA in a mixed solvent (acetone/isopropyl myristate, 4:1 v/v) on day 0, and then 20μL of 2% TMA was applied to the surface of both ears on days 5, 8, 11, 14, 17, 20, 23, and 26. Miquelianin or Dex was administered orally daily from day 5 to 26. |
Dosage form | 4mg/mL, 10mg/mL; po; 21d |
Applications | Oral administration of Miquelianin can significantly reduce serum IgE levels and the expression of Th2-related cytokines (such as IL-4 and IL-5) in the spleen, thereby inhibiting excessive Th2 immune responses. |
References: | |
| Cas No. | 22688-79-5 | SDF | |
| Synonyms | Miquelianin, Quercetin 3-O-β-Dglucuronide | ||
| Canonical SMILES | OC1=CC(O)=C(C(C(O[C@H]2[C@H](O)[C@@H](O)[C@H](O)[C@@H](C(O)=O)O2)=C(C3=CC=C(O)C(O)=C3)O4)=O)C4=C1 | ||
| Formula | C21H18O13 | M.Wt | 478.4 |
| Solubility | DMF: 30 mg/ml,DMSO: 20 mg/ml,Ethanol: 10 mg/ml,PBS (pH 7.2): 2 mg/ml | Storage | Store at 2-8°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 2.0903 mL | 10.4515 mL | 20.903 mL |
| 5 mM | 418.1 μL | 2.0903 mL | 4.1806 mL |
| 10 mM | 209 μL | 1.0452 mL | 2.0903 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 23 reference(s) in Google Scholar.)
GLPBIO products are for RESEARCH USE ONLY. Please make sure your review or question is research based.
Required fields are marked with *