CRISPR/Cas9
CRISPR/Cas9 (Clustered Regularly-Interspaced Short Palindromic Repeats/Cas9) is a bacterial immune system that has been adapted for genome editing in mammalian cells. Cas9 is a programmable nuclease that generates double-stranded breaks (DSB) in DNA dictated by binding of a ~20 nucleotide recombinant “guide RNA” (gRNA) to the target site. DSB’s produced by Cas9 are most often repaired through the cell’s error-prone non-homologous end joining (NHEJ) pathway, resulting in small insertions or deletions (indels). The vast majority of indels shift the reading frame, introducing a premature stop codon or resulting in nonsense mediated decay of the mRNA-effectively “knocking out” a gene.
The CRISPR/Cas9 system has substantially advanced efforts in specific gene editing and has been successfully applied to modify the episomal genomes of human and other organisms. The CRISPR/Cas9 system utilizes a prokaryotic RNA-guided programmable nuclease that can make a double-strand DNA break (DSB) at a specific site under the guidance of a leading RNA. This DSB process depends on the co-expression of two basic components: a guide RNA (gRNA) and Cas9 nuclease. Making a specific DSB can trigger DNA repair via either error-prone non-homologous end joining (NHEJ) or homology-directed repair (HDR). In the presence of the CRISPR/Cas9 system, the NHEJ inhibitor SCR7 is proven to increase the efficiency of Cas9-mediated HDR by at least by 7-fold in mammalian cells. Genome editing via CRISPR/Cas9 has become an efficient and reliable way to make precise, targeted changes to the genome of living cells.
Targets for CRISPR/Cas9
Products for CRISPR/Cas9
- Cat.No. Product Name Information
- GC64111 BRD0539 BRD0539 is a cell-permeable and non-toxic inhibitor of CRISPR-Cas9.
-
GC17683
Brefeldin A
Brefeldin A (BFA) is a fungal macrocyclic lactone and a potent, reversible inhibitor of intracellular vesicle formation and protein trafficking between the endoplasmic reticulum (ER) and the Golgi apparatus.
- GC15743 L-755,507 β3 adrenergic receptor agonist
- GC14075 Nocodazole A tubulin production inhibitor,anti-neoplastic agent
- GC11251 NU7441 (KU-57788) NU7441 (KU-57788) (NU7441) is a highly potent and selective DNA-PK inhibitor with an IC50 of 14 nM. NU7441 (KU-57788) is an NHEJ pathway inhibitor. NU7441 (KU-57788) also inhibits PI3K and mTOR with IC50s of 5.0 and 1.7 μM, respectively.
- GC16265 RS-1 RAD51 activator
- GC12106 SCR7 DNA ligase IV inhibitor
- GC10637 Zidovudine Reverse transcriptase inhibitor