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Resveratrol

Catalog No.GC14553

Resveratrol Chemical Structure

A SIRT1 activator

Size Price Stock Qty
10mM (in 1mL DMSO)
$38.00
In stock
200mg
$34.00
In stock
500mg
$54.00
In stock

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Sample solution is provided at 25 µL, 10mM.

Quality Control

Quality Control & SDS

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Protocol

Cell experiment [1]:

Cell lines

primary neuronal cultures; the neuroblastoma SH-SY5Y cell line

Preparation method

The solubility of this compound in DMSO is >9.7mg/mL. General tips for obtaining a higher concentration: Please warm the tube at 37℃ for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below -20℃ for several months.

Reacting condition

0.1, 1, and 10 μM; 50 μM

Applications

In primary neuronal cultures, resveratrol (0.1, 1, and 10 μM) inhibited OGD/reperfusion-induced apoptosis and inhibited the overexpression of caspase-3 and caspase-12 mRNA in a concentration-dependent way. In the neuroblastoma SH-SY5Y cell line, resveratrol (50 μM) inhibited excess dopamine-induced cell death by ameliorating intracellular oxidative stress and increasing the activity of prosurvival gene Bcl-2.

Animal experiment [2]:

Animal models

rats subjected to myocardial ischemia

Dosage form

2.5, 5.0, 25 or 50 mg/kg; fed for 14 days by gavaging

Application

In rats subjected to myocardial ischemia, resveratrol (2.5, 5.0 mg/kg) exhibited cardioprotection by improved post-ischemic ventricular recovery and reduction of myocardial infarct size and cardiomyocyte apoptosis. However, 25 or 50 mg/kg dose of resveratrol inhibited cardiac function and increased myocardial infarct size and number of apoptotic cells.

Other notes

Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal.

References:

[1]. Nilendra Singh, Megha Agrawal, and Sylvain Doré. Neuroprotective Properties and Mechanisms of Resveratrol in in Vitro and in Vivo Experimental Cerebral Stroke Models. ACS Chem Neurosci. 2013 Aug 21; 4(8): 1151–1162.

[2]. Dudley J1, Das S, Mukherjee S, et al. Resveratrol, a unique phytoalexin present in red wine, delivers either survival signal or death signal to the ischemic myocardium depending on dose. J Nutr Biochem. 2009 Jun;20(6):443-52.

Background

Resveratrol (trans-Resveratrol; SRT501), a natural polyphenolic phytoalexin that possesses anti-oxidant, anti-inflammatory, cardioprotective, and anti-cancer properties. Resveratrol (SRT 501) has a wide spectrum of targets including mTOR, JAK, β-amyloid, Adenylyl cyclase, IKKβ, DNA polymerase. Resveratrol also is a specific SIRT1 activator[1][2][3][4]. Resveratrol is a potent pregnane X receptor (PXR) inhibitor[5].

Resveratrol (trans-Resveratrol; SRT501) is one of the numerous polyphenolic compounds found in several vegetal sources In the vast majority of cases, Resveratrol displays inhibitory/activatory effects in the micromolar range, which is potentially attainable pharmacologically, although targets with affinities in the nanomolar range have also been reported[1]. MCF-7 cells are plated in DME-F12 medium supplemented with 5% FBS in the presence of increasing concentrations of Resveratrol. Control cells are treated with the same volume of vehicle only (0.1% ethanol). Resveratrol inhibits the growth of MCF-7 cells in a dose-dependent fashion. Addition of 10 μM Resveratrol results in an 82% inhibition of MCF-7 cell growth after 6 days while at 1 μM, only a 10% inhibition is observed. The cells treated with 10 μM Resveratrol have a doubling time of 60 hr whereas control cells doubled every 30 hr. Trypan blue exclusion assay shows that at concentrations of 10 μM or lower, Resveratrol does not affect cell viability (90% viable cells) whereas at 100 μM, only 50% of the cells are viable after 6 days of Resveratrol treatment. Moreover, MCF-7 cells do not undergo apoptosis after incubation with Resveratrol at concentration of 10 μM as determined by ApoAlert Annexin V Apoptosis kit[2].

The average tumor volume is reduced by treatment with Resveratrol (trans-Resveratrol; SRT501) at a dose of 50 mg/kg body weight (195.5±124.8 mm3; P<0.05) or 100 mg/kg body weight (81.7±70.5 mm3; P<0.001) compare with the vehicle-treated animals (315±94 mm3). There is a good correlation between the tumor volume and the tumor mass[3].

References:
[1]. Pirola L, et al. Resveratrol: one molecule, many targets. IUBMB Life. 2008 May;60(5):323-32.
[2]. Lu R, et al. Resveratrol, a natural product derived from grape, exhibits antiestrogenic activity and inhibits the growth of human breast cancer cells. J Cell Physiol. 1999 Jun;179(3):297-304.
[3]. Lee MH, et al. Resveratrol suppresses growth of human ovarian cancer cells in culture and in a murine xenograft model: eukaryotic elongation factor 1A2 as a potential target. Cancer Res. 2009 Sep 15;69(18):7449-58.
[4]. Du LL, et al. Activation of sirtuin 1 attenuates cerebral ventricular streptozotocin-induced tau hyperphosphorylation and cognitive injuries in rat hippocampi. Age (Dordr). 2014 Apr;36(2):613-23.
[5]. Smutny T, et al. Resveratrol as an inhibitor of pregnane X receptor (PXR): another lesson in PXR antagonism. J Pharmacol Sci. 2014;126(2):177-8.

Chemical Properties

Cas No. 501-36-0 SDF
Chemical Name 5-[(E)-2-(4-hydroxyphenyl)ethenyl]benzene-1,3-diol
Canonical SMILES C1=CC(=CC=C1C=CC2=CC(=CC(=C2)O)O)O
Formula C14H12O3 M.Wt 228.24
Solubility ≥ 9.65 mg/mL in DMSO, ≥ 48.2 mg/mL in EtOH with ultrasonic Storage Store at -20°C
General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.
Shipping Condition Evaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request

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