Name: Sappanone A
Brand: GlpBio
SKU: GC41626
Availability: InStock
Rating: 5 (4 reviews)

GlpBio Products Cited In Reputable Papers

Nature
610.7931 (2022): 366-372

Nature
618, 1017–1023 (2023)

Cell
183.7 (2020): 1867-1883

Cell Research
31, 1291–1307 (2021)

Cell Research
33, 273–287 (2023)

Cell Research
33, 546–561 (2023)

Molecular Cancer
21.1 (2022): 1-17

Molecular Cancer
21.1 (2022): 1-18

Cancer Cell
39 (2021): 1-16

Cell Host Microbe
30.8 (2022): 1124-1138

Cell Host Microbe
31.5 (2023): 766-780

Cell Host Microbe
31.3 (2023): 418-43

Cell Metabolism
34.2 (2022): 240-255

Ann Rheum Dis
82(4): 533-545 (2023)

Cell Mol Immunol
20, 264–276 (2023)

Adv Funct Mater
33.35 (2023): 2214998

Product Documents

Quality Control & SDS

View current batch:

Purity: >99.00%

Protocol

Cell experiment [1]:
Cell lines	Chondrocytes
Preparation Method	Cells were treated with 0, 5, 10, 20, or 40 µM of sappanone A for 24 h. Then, the cells were incubated for 2 h with 10 µl of CCK-8 solution, and the absorbance (450 nm) was detected.
Reaction Conditions	0-40µM；24h
Applications	40 µM sappanone A produced a significant inhibitory effect on cell viability. Concentrations of 5, 10, and 20 µM showed no significant change in cell viability.
Animal experiment [2]:
Animal models	Sprague Dawley rats
Preparation Method	The rats were divided into four groups (eight rats/group) as follows: (A) Normal group, (B) MI group, (C) MI + Curcumin 25 mg/kg group, (D) MI + Sappanone A 50 mg/kg group. The rats in groups A and B received vehicle (10% dimethyl sulfoxide, DMSO, and 90% polyethylene glycol, PEG400), and the rats in groups C and D were treated with 25 mg/kg curcumin, and 50 mg/kg sappanone A dissolved in vehicle by oral injection daily for 5 days.
Dosage form	50 mg/kg；p.o.；5 days
Applications	Sappanone A significantly improved left ventricular (LV) systolic and diastolic function in a rat myocardial ischemia/reperfusion injury model, especially in the early phase development of myocardial infarction.
References: [1]. Zhang Z, Zhang NZ, et,al. Sappanone A Alleviated IL-1β-Induced Inflammation in OA Chondrocytes through Modulating the NF-κB and Nrf2/HO-1 Pathways. Dis Markers. 2022 Sep 16;2022:2380879. doi: 10.1155/2022/2380879. PMID: 36157214; PMCID: PMC9507726. [2]. Jo W, Min BS, et,al.Sappanone A Prevents Left Ventricular Dysfunction in a Rat Myocardial Ischemia Reperfusion Injury Model. Int J Mol Sci. 2020 Sep 21;21(18):6935. doi: 10.3390/ijms21186935. PMID: 32967328; PMCID: PMC7555706.

Sappanone A is a homoisoflavanone with strong antioxidant and anti-inflammatory activities. It is one of the active ingredients of Sappan Lignum, it can inhibit microglia activation in the brain and has a neuroinflammatory effect[1-2].

Sappanone A(0-30µM；30min) inhibited the production of nitric oxide (NO), prostaglandin E2 (PGE2) and interleukin-6 (IL-6) as well as the expression of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2) and IL-6 in lipopolysaccharide (LPS)-stimulated RAW264.7 cells[3]. sappanone A (1.1 µM )significantly inhibited IBMX-induced increase of melanin content in B16 cells [4].40 µM sappanone A produced a significant inhibitory effect on cell viability. Concentrations of 5, 10, and 20 µM showed no significant change in cell viability. Sappanone A inhibited the IL-1β-stimulated production of NO, PGE2, iNOS, COX-2, TNF-α, IL-6, and IL-8 in OA chondrocytes[5].Sappanone A decreased cell viability of HOK cells and mouse salivary gland cells under ionising radiation[6].

Sappanone A (12.5, 25 and 50 mg/kg; 3days;i.p.) was found to attenuate the airway inflammation and mucus hypersecretion induced by the Ovalbumin(OVA) challenge in BALB/c mice[7]. Sappanone A(50 mg/kg；p.o.；5 days) significantly improved left ventricular (LV) systolic and diastolic function in a rat myocardial ischemia/reperfusion injury model, especially in the early phase development of myocardial infarction[8]. Sappanone A (10、20、40 mg/kg;i.p. ) reduced myocardial infarct size and the release of lactate dehydrogenase (LDH) and creatine kinase-MB (CK-MB) in a dose-dependent manner in myocardial ischemia reperfusion injury (MIRI) mice^[9].

References:
[1]. Kang L, Zhao H, et,al. Sappanone A protects mice against cisplatin-induced kidney injury. Int Immunopharmacol. 2016 Sep;38:246-51. doi: 10.1016/j.intimp.2016.05.019. Epub 2016 Jun 16. PMID: 27318179.
[2]. Syamsunarno MRA, Safitri R, et,al.Protective Effects of Caesalpinia sappan Linn. and Its Bioactive Compounds on Cardiovascular Organs. Front Pharmacol. 2021 Sep 15;12:725745. doi: 10.3389/fphar.2021.725745. PMID: 34603037; PMCID: PMC8479160.
[3]. Lee S, Choi SY, et,al.Sappanone A exhibits anti-inflammatory effects via modulation of Nrf2 and NF-κB. Int Immunopharmacol. 2015 Sep;28(1):328-36. doi: 10.1016/j.intimp.2015.06.015. Epub 2015 Jun 26. PMID: 26122134.
[4]. Chang TS, Chao SY, et,al.Melanogenesis inhibition by homoisoflavavone sappanone A from Caesalpinia sappan. Int J Mol Sci. 2012;13(8):10359-10367. doi: 10.3390/ijms130810359. Epub 2012 Aug 20. PMID: 22949866; PMCID: PMC3431864.
[5]. Zhang Z, Zhang NZ, et,al. Sappanone A Alleviated IL-1β-Induced Inflammation in OA Chondrocytes through Modulating the NF-κB and Nrf2/HO-1 Pathways. Dis Markers. 2022 Sep 16;2022:2380879. doi: 10.1155/2022/2380879. PMID: 36157214; PMCID: PMC9507726.
[6]. Zhao XF, Wang ZY, et,al.Sappanone A Aggrandises Ionising Radiation-induced Damage in Oral Epithelial Cells. Chin J Dent Res. 2022 Dec 8;25(4):261-267. doi: 10.3290/j.cjdr.b3628117. PMID: 36479890.
[7]. Liu X, Yu D, et,al.Sappanone A Attenuates Allergic Airway Inflammation in Ovalbumin-Induced Asthma. Int Arch Allergy Immunol. 2016;170(3):180-6. doi: 10.1159/000448331. Epub 2016 Aug 30. PMID: 27576536.
[8]. Jo W, Min BS, et,al.Sappanone A Prevents Left Ventricular Dysfunction in a Rat Myocardial Ischemia Reperfusion Injury Model. Int J Mol Sci. 2020 Sep 21;21(18):6935. doi: 10.3390/ijms21186935. PMID: 32967328; PMCID: PMC7555706.
[9].Shi X, Tao G, et,al. Sappanone A Protects Against Myocardial Ischemia Reperfusion Injury by Modulation of Nrf2. Drug Des Devel Ther. 2020 Jan 8;14:61-71. doi: 10.2147/DDDT.S230358. PMID: 32021092; PMCID: PMC6955610.

Cas No.	104778-14-5,102067-84-5	SDF
Canonical SMILES	OC1=CC=C(C(/C(CO2)=C/C3=CC=C(O)C(O)=C3)=O)C2=C1
Formula	C16H12O5	M.Wt	284.3
Solubility	DMF: 20 mg/ml,DMSO: 15 mg/ml,Ethanol: 5 mg/ml,PBS (pH 7.2): 0.25 mg/ml	Storage	Store at -20°C,protect from light
General tips	Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition	Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table

Prepare stock solution
		1 mg	5 mg	10 mg
	1 mM	3.5174 mL	17.5871 mL	35.1741 mL
	5 mM	0.7035 mL	3.5174 mL	7.0348 mL
	10 mM	0.3517 mL	1.7587 mL	3.5174 mL

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In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.

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