Serotonin HCl (Synonyms: 5-HT, 5-Hydroxytryptamine) |
| Catalog No.GC14843 |
Serotonin HCl a neurotransmitter, is a key messenger that mediates several central and peripheral functions in the human body.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 153-98-0
Sample solution is provided at 25 µL, 10mM.
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Serotonin HCl a neurotransmitter, is a key messenger that mediates several central and peripheral functions in the human body[1]. Serotonin HCl is also a catechol O-methyltransferase (COMT) inhibitor with a Ki of 44 µM[2].
In vitro, Serotonin HCl (1 nmol/L-10 micromol/L) caused vasoconstriction in a concentration-dependent manner[3]. In vitro, 10 μM Serotonin HCl proliferates colon cancer cells through enhancing YAP (Yes-associated protein) expression[1]. In vitro, for left lower lobe of lungs isolated from the dog, infusion of Serotonin HCl (100 micrograms/min) increased the pulmonary pre- and post-capillary resistances, but did not change the filtration coefficient[4].
In vivo, topical Serotonin HCl (1-10 nmols) or the Serotonin HCl1/7 receptor agonist, 5-carboxamidotryptamine (10-30 nM), dilated third- and fourth-order arterioles, responses that were abolished by 1 μM SB269970, a selective Serotonin HCl7 receptor antagonist[5].
References:
[1] Yu H, et al. Serotonin acts through YAP to promote cell proliferation: mechanism and implication in colorectal cancer progression. Cell Commun Signal. 2023 Apr 12;21(1):75.
[2] Tsao D, et al. Serotonin-induced hypersensitivity via inhibition of catechol O-methyltransferase activity. Mol Pain. 2012 Apr 13;8:25.
[3] Tanaka N, et al. Both 5-hydroxytryptamine 5-HT2A and 5-HT1B receptors are involved in the vasoconstrictor response to 5-HT in the human isolated internal thoracic artery. Clin Exp Pharmacol Physiol. 2008 Jul;35(7):836-40.
[4] Sumita T, et al. 5-Hydroxytryptamine receptor subtypes participating in pulmonary edema in dogs. Eur J Pharmacol. 1989 May 2;164(1):69-75.
[5] Jackson WF, et al. 5-HT7 receptors mediate dilation of rat cremaster muscle arterioles in vivo.Microcirculation. 2023 Aug;30(5-6):e12808.
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Cell experiment: |
For macrophage culture, peritoneal cavity cells are collected from WT and IL-13-/- mice with or without dextran sodium sulfate (DSS) treatment and cultured. Cells are plated at a concentration of 3.0×106 cells per millilitre, and treated with either lipopolysaccharides (LPS); (100 ng/mL) or Serotonin hydrochloride (10-10 M) for 24 hours. The culture supernatant are collected and stored in -80°C until determination of cytokine levels using protein array system[2]. |
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Animal experiment: |
Dextran sodium sulfate (DSS) colitis is induced by orally administering 5% DSS in drinking water for 5 days. In a separate experiment, IL-13-/- mice are injected subcutaneously with 100 mg/kg of Serotonin hydrochloride (5-HTP) twice daily for 8 days beginning 3 days prior to induction of DSS colitis; whereas, the control IL-13-/- mice receive saline as vehicle. Animals are anaesthetized prior to euthanization via cervical dislocation at the conclusion of each experiment or if they reach a predetermined end point (ie, loss of ≥20% body weight and/or significant deterioration of body condition)[2]. |
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References: [1]. Tsao D, et al. Serotonin-induced hypersensitivity via inhibition of catechol O-methyltransferase activity. Mol Pain. 2012 Apr 13;8:25. |
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| Cas No. | 153-98-0 | SDF | |
| Synonyms | 5-HT, 5-Hydroxytryptamine | ||
| Chemical Name | 3-(2-aminoethyl)-1H-indol-5-ol;hydrochloride | ||
| Canonical SMILES | C1=CC2=C(C=C1O)C(=CN2)CCN.Cl | ||
| Formula | C10H12N2O.HCl | M.Wt | 212.68 |
| Solubility | DMF: 16 mg/ml,DMSO: 10 mg/ml,Ethanol: 1.2 mg/ml,PBS (pH 7.2): 5 mg/ml | Storage | Store at 2-8°C, protect from light |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 4.7019 mL | 23.5095 mL | 47.019 mL |
| 5 mM | 0.9404 mL | 4.7019 mL | 9.4038 mL |
| 10 mM | 0.4702 mL | 2.3509 mL | 4.7019 mL |
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Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
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Quality Control & SDS
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- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 29 reference(s) in Google Scholar.)
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