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Sodium Palmitate (Synonyms: Hexadecanoic acid sodium salt, Palmitic acid sodium salt )

Catalog No.GC19521

Sodium palmitate is a common saturated fatty acid produced during the process of fatty acid synthesis.

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Sodium Palmitate Chemical Structure

Cas No.: 408-35-5

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$21.00
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10g
$34.00
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Sample solution is provided at 25 µL, 10mM.

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Description of Sodium Palmitate

Sodium palmitate is a common saturated fatty acid produced during the process of fatty acid synthesis. In the field of life science research, sodium palmitate is primarily used for studying cell metabolism, lipid research, and as a nutritional supplement in cell culture. Sodium palmitate can enhance lipogenesis and lipid droplet formation in various cell lines, and it is used to establish cell models of lipid droplet accumulation.

In vitro, Sodium palmitate (0.1, 0.25, or 0.5 mM; 12-72 h) can increase the mRNA levels of Notch1, -2, and -4 in LX2, Huh7, and MIHA liver cell lines[1]. Sodium palmitate can induce the expression of Glucose Regulated Protein 78 (GRP 78) and CCAAT/Enhancer Binding Protein Homologous Protein (CHOP) in mouse granule cells[2]. Moreover, treating LO2 cells with 100 µmol/l sodium palmitate for 48 hours significantly inhibits cell viability, and this concentration and incubation time can be used as a model of lipotoxicity in experiments[3].

References:

[1] Wen-Jin Ding, et al. Expression of Notch family is altered in non‑alcoholic fatty liver disease. Mol Med Rep. 2020 Sep;22(3):1702-1708.

[2] Harada H, et al. Antitumor activity of palmitic acid found as a selective cytotoxic substance in a marine red alga. Anticancer Res. 2002 Sep-Oct;22(5):2587-90.

[3] Ma, Cheng, Yuan, et al. Lipid storage droplet protein 5 reduces sodium palmitateinduced lipotoxicity in human normal liver cells by regulating lipid metabolismrelated factors[J]. Molecular Medicine Reports, 2019, 20(2):879-886.

Protocol of Sodium Palmitate

Cell experiment [1]:

Cell lines

LX2, Huh7 and MIHA liver cell lines

Preparation method

PA was dissolved in DMEM containing 1% BSA and filtered through a 0.22-µm filter, then added to the cells and incubated at 37°C to 80% confluency in DMEM with 10% FBS. The concentrations of PA used were 0.1, 0.25 or 0.5 mM, and the incubation times were 12–72 h.

Reaction Conditions

0.1, 0.25, 0.5 mM;12-72 h

Applications

Sodium palmitate can increase the mRNA levels of Notch1, -2, and -4 in LX2, Huh7, and MIHA liver cell lines.

Cell experiment [2]:

Cell lines

LO2 cells

Preparation method

cells were treated with sodium palmitate at 25, 50, 75, 100, 125 or 150 µmol/L and incubated for 12, 24 or 48 h. Following incubation, CCK-8 reagent was added into each well and the cells were incubated in humidified environment with 5% CO2 at 37°C for 2 h.

Reaction Conditions

25, 50, 75, 100, 125 or 150 µmol/L; 12, 24 or 48 h

Applications

Treating LO2 cells with greater than 100 µmol/l sodium palmitate for 48 h significantly inhibited cell viability and can be used as a lipotoxicity model in experiments.

References:

[1] Wen-Jin Ding, et al. Expression of Notch family is altered in non‑alcoholic fatty liver disease. Mol Med Rep. 2020 Sep;22(3):1702-1708.

[2]Ma, Cheng, Yuan, et al. Lipid storage droplet protein 5 reduces sodium palmitateinduced lipotoxicity in human normal liver cells by regulating lipid metabolismrelated factors[J]. Molecular Medicine Reports, 2019, 20(2):879-886.

Chemical Properties of Sodium Palmitate

Cas No. 408-35-5 SDF
Synonyms Hexadecanoic acid sodium salt, Palmitic acid sodium salt
Formula C16H31NaO2 M.Wt 278.41
Solubility Soluble in methanol; DMSO: 8.33 mg/mL (29.92 mM; ultrasonic and warming and adjust pH to 4 with 1M HCl and heat to 60°C) Storage Store at 2-8°C
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table of Sodium Palmitate

Prepare stock solution
1 mg 5 mg 10 mg
1 mM 3.5918 mL 17.9591 mL 35.9183 mL
5 mM 0.7184 mL 3.5918 mL 7.1837 mL
10 mM 0.3592 mL 1.7959 mL 3.5918 mL
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In vivo Formulation Calculator (Clear solution) of Sodium Palmitate

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

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Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.

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Average Rating: 5 ★★★★★ (Based on Reviews and 39 reference(s) in Google Scholar.)

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