(±)8-Amino-7-Oxononanoic Acid (hydrochloride) (Synonyms: (±)8-KAPA) |
| Catalog No.GC91571 |
(±)8-Amino-7-Oxononanoic acid ((±)8-KAPA) is a racemic mixture of 8(R)-KAPA and the vitamer of biotin 8(S)-KAPA .
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 21286-96-4
Sample solution is provided at 25 µL, 10mM.
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(±)8-Amino-7-Oxononanoic acid ((±)8-KAPA) is a racemic mixture of 8(R)-KAPA and the vitamer of biotin 8(S)-KAPA .[1],[2] It is biosynthesized as the 8(S) form but racemizes in culture media in a pH-dependent manner, with a higher racemization rate under very low (pH <2), physiological, and basic pH conditions.[2] 8(S)-KAPA increases proliferation in S. cerevisiae in a concentration-dependent manner and is an intermediate in the biosynthesis of biotin in microbes. 8(S)-KAPA, but not 8(R)-KAPA, is a substrate for E. coli and M. tuberculosis 7,8-diaminopelargonic acid aminotransferase (DAPA AT), which leads to accumulation of 8(R)-KAPA in M. tuberculosis cultures after exogenous application of racemic KAPA.
References:
[1].Lucet, D., Le Gall, T., Mioskowski, C., et al.First synthesis of both enantiomers of the biotin vitamer 8-amino-7-oxopelargonic acidTetrahedron-Asymmetr.7(4)985-988(1996).
[2].Mann, S., Colliandre, L., Labesse, G., et al.Inhibition of 7,8-diaminopelargonic acid aminotransferase from Mycobacterium tuberculosis by chiral and achiral anologs of its substrate: biological implicationsBiochimie91(7)826-834(2009).
| Cas No. | 21286-96-4 | SDF | |
| Sinónimos | (±)8-KAPA | ||
| Formula | C9H17NO3•HCl | M.Wt | 223.7 |
| Solubility | DMSO: Sparingly Soluble: 1-10 mg/ml,Ethanol: Slightly Soluble: 0.1-1 mg/ml,PBS (pH 7.2): Sparingly Soluble: 1-10 mg/ml | Storage | -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 4.4703 mL | 22.3514 mL | 44.7027 mL |
| 5 mM | 0.8941 mL | 4.4703 mL | 8.9405 mL |
| 10 mM | 0.447 mL | 2.2351 mL | 4.4703 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
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Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >95.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)
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