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2-(Methylamino)-1H-purin-6(7H)-one (N2-methylguanine)

Catalog No.GC33496

La 2-(metilamino)-1H-purina-6(7H)-ona (N2-metilguanina) (N2-metilguanina) es un nucleÓsido modificado.

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2-(Methylamino)-1H-purin-6(7H)-one (N2-methylguanine) Chemical Structure

Cas No.: 10030-78-1

Tamaño Precio Disponibilidad Cantidad
1mg
47,00 $
Disponible
5mg
117,00 $
Disponible
10mg
180,00 $
Disponible
50mg
450,00 $
Disponible

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Sample solution is provided at 25 µL, 10mM.

Description of 2-(Methylamino)-1H-purin-6(7H)-one (N2-methylguanine)

N2-Methylguanine is a modified nucleoside. N2-Methylguanine is an endogenous methylated nucleoside found in human fluids.

N2-methylguanine is found within both helical and looped regions of RNA secondary structure, and it can exist in either the s-cis or the s-trans rotamer. If there is a rotational preference for the methyl group, the effect of N2-methylguanine substitution may be specific to the sequence context depending upon which face of the base participates in hydrogen bonding[1]. N2-methylguanine is the principal kinetic barrier for reverse transcription in the 1340 bases proximal to the 5' end of E. coli 16S rRNA. Transcription intermediates correspond to attenuation at the positions of N2-methylguanine in the rRNA sequence. The relaxation time for elongation of the cDNA through m2G is approximately 3 min[2].

[1]. Rife JP, et al. N 2-methylguanosine is iso-energetic with guanosine in RNA duplexes and GNRA tetraloops. Nucleic Acids Res. 1998 Aug 15;26(16):3640-4. [2]. Youvan DC, et al. Reverse transcriptase pauses at N2-methylguanine during in vitro transcription of Escherichia coli 16S ribosomal RNA. Proc Natl Acad Sci U S A. 1979 Aug;76(8):3751-4.

Chemical Properties of 2-(Methylamino)-1H-purin-6(7H)-one (N2-methylguanine)

Cas No. 10030-78-1 SDF
Canonical SMILES O=C1N=C(NC)NC2=C1NC=N2
Formula C6H7N5O M.Wt 165.15
Solubility DMSO : < 1 mg/mL (insoluble or slightly soluble) Storage Store at -20°C
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table of 2-(Methylamino)-1H-purin-6(7H)-one (N2-methylguanine)

Prepare stock solution
1 mg 5 mg 10 mg
1 mM 6.0551 mL 30.2755 mL 60.551 mL
5 mM 1.211 mL 6.0551 mL 12.1102 mL
10 mM 0.6055 mL 3.0276 mL 6.0551 mL
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In vivo Formulation Calculator (Clear solution) of 2-(Methylamino)-1H-purin-6(7H)-one (N2-methylguanine)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.

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Average Rating: 5 ★★★★★ (Based on Reviews and 17 reference(s) in Google Scholar.)

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