DC-Chol (hydrochloride) (Synonyms: Cholesteryl 3β-N-(dimethylaminoethyl)carbamate) |
| Catalog No.GC43385 |
DC-Chol (hydrochloride) puede inhibir la formación de fibras de Aβ40, DC-Chol (ácido clorhídrico) puede inhibir la formación de amiloide de hCT oxidado.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 166023-21-8
Sample solution is provided at 25 µL, 10mM.
DC-Chol (hydrochloride) puede inhibir la formación de fibras de Aβ40, DC-Chol (ácido clorhídrico) puede inhibir la formación de amiloide de hCT oxidado.
Para DC-Chol (hidrocloruro), se observaron fibras de Aβ en la imagen de Aβ40 (30 µM) coincubado con una baja concentración de DC-Chol (hidrocloruro) (50 ng/mL). Sin embargo, en presencia de 500 µg/mL de DC-Chol (hidrocloruro), no se encontraron estructuras de fibras en la imagen[1]. Los liposomas catiónicos de DC-Chol/DOPE condensan y compactan adecuadamente el ADN CT mediante una fuerte interacción electrostática superficial impulsada entrópicamente[3]. Se han analizado lipoplexes constituidos por ADN de timo de ternera (CT-DNA) y liposomas catiónicos mixtos que consisten en proporciones variables del lípido catiónico 3β-[N-(N',N'-dimetilaminoetano)-carbamoil]colesterol hidrocloruro (DC-Chol (hidrocloruro)) y DOPE. Los liposomas DC-Chol/DOPE, con un diámetro hidrodinámico medio de alrededor de (120±10) nm, compactan y condensan fragmentos de ADN en sus superficies catiónicas mediante una fuerte interacción electrostática impulsada entrópicamente[4]. Las formulaciones preparadas con 50 mol% de DODAC o DC-Chol (hidrocloruro), y 20 mol% de DSPE-PEG(2000) exhibieron vidas medias de circulación que varían de 6,5 a 12,5 h. Las formulaciones de DC-Chol (hidrocloruro) preparadas con DSPE-PEG(2000) se acumularon tres veces más en tumores HT29 s.c. que su contraparte sin PEG[6]. DC-Chol (hidrocloruro) es internalizado a través de macrocitosis y endocitosis mediada por clatrina[5].
References:
[1]. Elbassal EA, Liu H, et,al. Effects of Charged Cholesterol Derivatives on Aβ40 Amyloid Formation. J Phys Chem B. 2016 Jan 14;120(1):59-68. doi: 10.1021/acs.jpcb.5b09557. Epub 2015 Dec 23. PMID: 26652010; PMCID: PMC4959543.
[2]. Lantz R, Busbee B, et,al. Effects of disulfide bond and cholesterol derivatives on human calcitonin amyloid formation. Biopolymers. 2020 May;111(5):e23343. doi: 10.1002/bip.23343. Epub 2019 Dec 5. PMID: 31804717; PMCID: PMC9254112.
[3]. RodrÍguez-Pulido A, Ortega F, et,al. A physicochemical characterization of the interaction between DC-Chol/DOPE cationic liposomes and DNA. J Phys Chem B. 2008 Oct 2;112(39):12555-65. doi: 10.1021/jp804066t. Epub 2008 Aug 27. PMID: 18729499.
[4]. MuÑoz-Ubeda M, RodrÍguez-Pulido A, et,al. Effect of lipid composition on the structure and theoretical phase diagrams of DC-Chol/DOPE-DNA lipoplexes. Biomacromolecules. 2010 Dec 13;11(12):3332-40. doi: 10.1021/bm1008124. Epub 2010 Nov 8. PMID: 21058732.
[5]. Rapaka H, Manturthi S, et,al. Effect of Methylation of the Hydrophilic Domain of Tocopheryl Ammonium-Based Lipids on their Nucleic Acid Delivery Properties. ACS Omega. 2022 Apr 29;7(18):15396-15403. doi: 10.1021/acsomega.1c06889. PMID: 35571792; PMCID: PMC9096827.
[6]. Ho EA, Ramsay E, et,al. Characterization of cationic liposome formulations designed to exhibit extended plasma residence times and tumor vasculature targeting properties. J Pharm Sci. 2010 Jun;99(6):2839-53. doi: 10.1002/jps.22043. PMID: 20091826.
| Microscopía de Fuerza Atómica (AFM) [1]: | |
Sustrato | Aβ40 |
Método de Preparación | Aβ40 (30 μM) en un tampón fosfato de pH 7.4 incubado en un mezclador Speci-Mix. Aβ40 en presencia de 50 ng/mL de colesterol-SO4, 500 μg/mL de colesterol-SO4, 50 ng/mL de DC-Col (hidrocloruro) y 500 μg/mL de DC-colesterol. |
Condiciones de Reacción | 50/500 ng/mL de DC-Col (hidrocloruro) con Aβ40 durante 17 h. |
Áreas de aplicación | Para DC-Col (hidrocloruro), se observaron fibras de Aβ en la imagen de Aβ40 (30 μM) coincubado con una baja concentración de DC-colesterol (50 ng/mL). Sin embargo, en presencia de 500 μg/mL de DC-colesterol, no se encontraron estructuras de fibras en la imagen. |
Referencias: [1]. Elbassal EA, Liu H, et, al. Effects of Charged Cholesterol Derivatives on Aβ40 Amyloid Formation. J Phys Chem B. 2016 Jan 14;120(1):59-68. doi: 10.1021/acs.jpcb.5b09557. Epub 2015 Dec 23. PMID: 26652010; PMCID: PMC4959543. | |
| Cas No. | 166023-21-8 | SDF | |
| Sinónimos | Cholesteryl 3β-N-(dimethylaminoethyl)carbamate | ||
| Chemical Name | (3β)-cholest-5-en-3-ol 3-[N-[2-(dimethylamino)ethyl]carbamate], monohydrochloride | ||
| Canonical SMILES | CC(C)CCC[C@@H](C)[C@@]1([H])CC[C@@]2([H])[C@]3([H])CC=C4C[C@@H](OC(NCCN(C)C)=O)CC[C@]4(C)[C@@]3([H])CC[C@@]21C.Cl | ||
| Formula | C32H56N2O2•HCl | M.Wt | 537.3 |
| Solubility | 10mg/mL in methanol, 5mg/mL in DMSO | Storage | Store at -20°C, protect from light |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.8612 mL | 9.3058 mL | 18.6116 mL |
| 5 mM | 0.3722 mL | 1.8612 mL | 3.7223 mL |
| 10 mM | 0.1861 mL | 0.9306 mL | 1.8612 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
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Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
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- Purity: >97.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 38 reference(s) in Google Scholar.)
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