DDX58 Protein, Human (His)

DDX58 protein serves as an innate immune receptor crucial for detecting cytoplasmic viral nucleic acids, initiating a downstream signaling cascade that leads to the production of type I interferons and pro-inflammatory cytokines. It forms a ribonucleoprotein complex with viral RNAs, promoting homooligomerization into filaments. This oligomerization facilitates the recruitment of RNF135, an E3 ubiquitin-protein ligase, which amplifies RIG-I-mediated antiviral signaling. Upon activation, DDX58 associates with MAVS/IPS1, activating TBK1 and IKBKE kinases, leading to the phosphorylation of IRF3 and IRF7. This activation prompts the transcription of antiviral immunological genes, including interferons IFN-alpha and IFN-beta. DDX58 recognizes various viral RNA ligands, such as 5'-triphosphorylated ssRNAs and dsRNAs, with the 5'-triphosphate moiety and blunt-end base pairing being crucial for its activity. The protein detects a broad range of RNA viruses and plays a role in antiviral signaling against dsDNA-containing viruses, such as Epstein-Barr virus. Additionally, it participates in granulocyte production, differentiation, bacterial phagocytosis, and the regulation of cell migration.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.