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Dehydroepiandrosterone (DHEA) (Synonyms: Androstenolone, trans-Dehydroandrosterone, DHEA, Diandrone, NSC 9896, Prasterone, Psicosterone)

Catalog No.GC11070

La dehidroepiandrosterona (DHEA) y su éster sulfato, DHEAS, juntos representan las hormonas esteroides más abundantes en el cuerpo humano.

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Dehydroepiandrosterone (DHEA) Chemical Structure

Cas No.: 53-43-0

Tamaño Precio Disponibilidad Cantidad
10mM (in 1mL DMSO)
36,00 $
Disponible
10g
69,00 $
Disponible
25g
150,00 $
Disponible

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Sample solution is provided at 25 µL, 10mM.

Product Documents

Quality Control & SDS

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Protocol

Cell experiment [1]:

Cell lines

HCC1 cell line

Preparation Method

Transferred cells to serum-free dehydroepiandrosterone (DHEA) containing either 0.1% ethanol which was added to all control cultures or 10-12-10-6 M DHEA. The cell culture supernatant was harvested after 72 h.

Reaction Conditions

10-12-10-6 M for 72 hours

Applications

Co-treatment with DEX (10-7 M)/ANDI (10-7 M) or DEX (10-7 M)/DHEA (10-7 M) reversed the increase in RANKL mRNA expression

Animal experiment [2]:

Animal models

female BALB/c mice

Preparation Method

Effects of treatment with dehydroepiandrosterone (DHEA) were assessed on either ovaries with functional corpora lutea (CL) or regressing CL by two s.c. injections of 60 mg DHEA/kg body weight (DHEA group), 24 h apart on days 3 and 4 after ovulation, followed by decapitation on day 5 (functional CL) or on day 7 and 8, followed by decapitation on day 9 (regressing CL).

Dosage form

s.c., 60 mg/kg

Applications

In mice with functional CL (day 5), the hyperandrogenization with dehydroepiandrosterone (DHEA) decreased both serum P and estradiol (E2) levels when compared to controls

References:

[1]: Harding G, Mak YT, Evans B, Cheung J, MacDonald D, Hampson G: The effects of dexamethasone and dehydroepiandrosterone (DHEA) on cytokines and receptor expression in a human osteoblastic cell line: potential steroid-sparing role of DHEA. Cytokine. 2006, 36: 57-68. 10.1016/j.cyto.2006.10.012.
[2]: Sander, V.A., Facorro, G.B., Piehl, L., Rubin de Celis, E., & Motta, A.B. Effect of DHEA and metformin on corpus luteum in mice. Reproduction. 138, 571-579 (2009).

Background

La dehidroepiandrosterona (DHEA) y su éster sulfato, DHEAS, juntos representan las hormonas esteroides más abundantes en el cuerpo humano [1].

El dehidroepiandrosterona (DHEA) (10-8 y 10-6 M) o DHEAS tratados previamente en cultivos corticales cerebrales de ratas aumentaron la supervivencia neuronal cuando los cultivos fueron sometidos a anoxia durante 2 h [2]. Cuando se sometieron cultivos corticales cerebrales de ratas a anoxia durante 2 h en una cámara anaeróbica y se trataron previamente con dehidroepiandrosterona (DHEA) (10-8 y 10-6 M) o DHEAS (10-6 M), hubo un aumento en la supervivencia neuronal. Utilizando precursores neurales cultivados del cerebro embrionario de rata, la dehidroepiandrosterona (DHEA) (50 y 100 nM) activó la proteína quinasa serina-treonina Akt, que está ampliamente implicada en señalización para la supervivencia celular [3].

El tratamiento con dehidroepiandrosterona (DHEA) mejoró significativamente la recuperación locomotora en ratones hembra CD-1 después de una lesión contusiva en la médula espinal (SCI), así como también la coordinación izquierda-derecha y el control motor fino, en comparación con los animales de control [4]. Los ratones tratados con dehidroepiandrosterona (DHEA) también presentaron una cantidad significativamente mayor de materia blanca preservada en el epicentro de la lesión y un área reducida de gliosis reactiva alrededor del sitio lesionado. El tratamiento con Dehidroepiandrosterona (DHEA) fue intensivo y consistió en tres modos diferentes de administración: un parche Matrigel DHEA (10-10 M) aplicado a la médula espinal antes del cierre de la herida, seguido por 12 días de inyecciones i.p. De solución salina que contenía Dehidroepiandrosterona (DHEA) (10-6 M o 0,02 mg/kg/día) después del SCI, y Dehidroepiandrosterona (DHEA) (10-6 M) en el agua potable durante 42 días [4].

References:
[1]. Rice SP, Zhang L, Grennan-Jones F, Agarwal N, Lewis MD, Rees DA, Ludgate M: Dehydroepiandrosterone (DHEA) treatment in vitro inhibits adipogenesis in human omental but not subcutaneous adipose tissue. Mol Cell Endocrinol 2010, 320: 51-57. 10.1016/j.mce.2010.02.017
[2]. C.E. Marx, L.F. Jarskog, J.M. Lauder, J.H. Gilmore, J.A. Lieberman, A.L. Morrow. Neurosteroid modulation of embryonic neuronal survival in vitro following anoxia. Brain Res., 871 (2000), pp. 104-112
[3]. L. Zhang, B. Li, W. Ma, J.L. Barker, Y.H. Chang, W. Zhao, D.R. Rubinow. Dehydroepiandrosterone (DHEA) and its sulfated derivative (DHEAS) regulate apoptosis during neurogenesis by triggering the Akt signaling pathway in opposing ways. Brain Res. Mol. Brain Res., 98 (2002), pp. 58-66
[4]. C. Fiore, D.M. Inman, S. Hirose, L.J. Noble, T. Igarashi, N.A. Compagnone. Treatment with the neurosteroid dehydroepiandrosterone promotes recovery of motor behavior after moderate contusive spinal cord injury in the mouse. J. Neurosci. Res., 75 (2004), pp. 391-400

Chemical Properties

Cas No. 53-43-0 SDF
Sinónimos Androstenolone, trans-Dehydroandrosterone, DHEA, Diandrone, NSC 9896, Prasterone, Psicosterone
Chemical Name (3S,8R,9S,10R,13S,14S)-3-hydroxy-10,13-dimethyl-1,2,3,4,7,8,9,11,12,14,15,16-dodecahydrocyclopenta[a]phenanthren-17-one
Canonical SMILES CC12CCC3C(C1CCC2=O)CC=C4C3(CCC(C4)O)C
Formula C19H28O2 M.Wt 288.42
Solubility ≥ 13.7 mg/mL in DMSO, ≥ 58.6 mg/mL in ETOH Storage Store at RT
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table

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1 mg 5 mg 10 mg
1 mM 3.4672 mL 17.3358 mL 34.6717 mL
5 mM 0.6934 mL 3.4672 mL 6.9343 mL
10 mM 0.3467 mL 1.7336 mL 3.4672 mL
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