Inicio>>Natural Products>>Diphenylterazine (DTZ)

Diphenylterazine (DTZ) (Synonyms: DTZ)

Catalog No.GC33428

Difenilterazina (DTZ) es un agente de bioluminiscencia.

Products are for research use only. Not for human use. We do not sell to patients.

Diphenylterazine (DTZ) Chemical Structure

Cas No.: 344940-63-2

Tamaño Precio Disponibilidad Cantidad
1mg
105,00 $
Disponible
5mg
210,00 $
Disponible
10mg
329,00 $
Disponible
50mg
686,00 $
Disponible

Tel:(909) 407-4943 Email: sales@glpbio.com

Reseñas de cliente

Based on customer reviews.

  • GlpBio Citations

    GlpBio Citations
  • Bioactive Compounds Premium Provider

    Bioactive Compounds Premium Provider

Sample solution is provided at 25 µL, 10mM.

Product Documents

Quality Control & SDS

View current batch:

Protocol

Cell experiment [1]:

Cell lines

HEK 293T

Preparation Method

Cell viability was determined using RealTime-Glo MT Cell Viability Assay (Promega) after incubation of HEK 293T cells with individual luciferin substrates for 24 h at 37 °C. Cell morphology was further evaluated using microscopy.

Reaction Conditions

24h 30 μM Diphenylterazine

Applications

Compared to other substrates,As a novel luciferase substrate based on CTZ analogue, Diphenylterazine has bright red-shift bioluminescence. Moreover, Diphenylterazine alone yielded very little background and thus exhibited excellent signal-to-background ratios , Furthermore, Diphenylterazine elicited minimal cell toxicity at millimolar concentrations.

Animal experiment [2]:

Animal models

BALB/c mice

Preparation Method

Bioluminescence imaging of intravenously injected HEK 293T cells:BALB/c mice are used and transfected with cells expressing biotinase gene by injecting cells into the tail vein of BALB/c mice. After the diminishing of the bioluminescence, 0.3 µmol Diphenylterazine is intraperitoneally injected. Mice are imaged with a 1-min exposure per frame over a course of 20 min.

Dosage form

0.3 µmol Diphenylterazine

Applications

Diphenylterazine was used as an emerging luciferase reporter substrate in mice. The imaging of deep tissue targets in mice was evaluated by injecting luciferase gene expressing HEK 293T cells through the tail vein. First, Diphenylterazine as a substrate has no background in control mice, and when injected into mice transfected with the luciferase gene, the fluorescence emitted by Diphenylterazine can be detected, and the intensity of Diphenylterazine has increased significantly compared with other substrates.

References:

[1]. Yeh HW, Karmach O, Ji A, Carter D, Martins-Green MM, Ai HW. Red-shifted luciferase-luciferin pairs for enhanced bioluminescence imaging. Nat Methods. 2017 Oct;14(10):971-974. doi: 10.1038/nmeth.4400. Epub 2017 Sep 4. PMID: 28869756; PMCID: PMC5678970.

Background

Difenilterazina, un análogo de CTZ, es un buen sustrato para la luciferasa. La difenilterazina es un agente de bioluminiscencia. La difenilterazina por sí sola produce muy poco fondo, lo que conduce a excelentes relaciones señal-ruido. Además, la difenilterazina provoca una toxicidad celular mínima a concentraciones milimolares. La difenilterazina podría sintetizarse a partir de reactivos comerciales económicos en dos pasos con rendimientos excelentes[1].

En experimentos celulares, podemos observar que Diphenylterazine tiene una relación señal-ruido más baja que otros sustratos, y Diphenylterazine causa una citotoxicidad mínima a concentraciones milimolares.

En contraste, otros sustratos probados indujeron la muerte celular dentro del rango de concentración del sustrato probado.

En el experimento con ratones, la bioluminiscencia del sitio de superficie, a una concentración de sustrato de 0.1 mM, la luminosidad del Diphenylterazine fue mayor que la del grupo control.

Lo mismo fue cierto para la imagen de objetivos de tejidos profundos, y la inyección de Diphenylterazine en ratones BALB/c no transfundidos no produjo ninguna emisión de fondo, y la fluorescencia todavía era detectable cuando se inyectaba por vía intravenosa en los ratones[2].

References:
[1].Tian X, Zhang Y, Li X, Xiong Y, Wu T, Ai HW. A luciferase prosubstrate and a red bioluminescent calcium indicator for imaging neuronal activity in mice. Nat Commun. 2022 Jul 8;13(1):3967. doi: 10.1038/s41467-022-31673-x. PMID: 35803917; PMCID: PMC9270435.
[2]. Yeh HW, et al. Red-shifted luciferase-luciferin pairs for enhanced bioluminescence imaging. Nat Methods. 2017 Oct;14(10):971-974.

Chemical Properties

Cas No. 344940-63-2 SDF
Sinónimos DTZ
Canonical SMILES O=C1C(CC2=CC=CC=C2)=NC3=C(C4=CC=CC=C4)NC(C5=CC=CC=C5)=CN31
Formula C25H19N3O M.Wt 377.44
Solubility DMF : 11 mg/mL Storage Store at -20°C, protect from light
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table

Prepare stock solution
1 mg 5 mg 10 mg
1 mM 2.6494 mL 13.2471 mL 26.4943 mL
5 mM 0.5299 mL 2.6494 mL 5.2989 mL
10 mM 0.2649 mL 1.3247 mL 2.6494 mL
  • Molarity Calculator

  • Dilution Calculator

Mass
=
Concentration
x
Volume
x
MW*
 
 
 
**When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / CoA (available online).

Calculate

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.

Reseñas

Review for Diphenylterazine (DTZ)

Average Rating: 5 ★★★★★ (Based on Reviews and 4 reference(s) in Google Scholar.)

5 Star
100%
4 Star
0%
3 Star
0%
2 Star
0%
1 Star
0%
Review for Diphenylterazine (DTZ)

GLPBIO products are for RESEARCH USE ONLY. Please make sure your review or question is research based.

Required fields are marked with *

You may receive emails regarding this submission. Any emails will include the ability to opt-out of future communications.