Diphenylterazine (DTZ) (Synonyms: DTZ) |
Catalog No.GC33428 |
Difenilterazina (DTZ) es un agente de bioluminiscencia.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 344940-63-2
Sample solution is provided at 25 µL, 10mM.
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Cell experiment [1]: | |
Cell lines |
HEK 293T |
Preparation Method |
Cell viability was determined using RealTime-Glo MT Cell Viability Assay (Promega) after incubation of HEK 293T cells with individual luciferin substrates for 24 h at 37 °C. Cell morphology was further evaluated using microscopy. |
Reaction Conditions |
24h 30 μM Diphenylterazine |
Applications |
Compared to other substrates,As a novel luciferase substrate based on CTZ analogue, Diphenylterazine has bright red-shift bioluminescence. Moreover, Diphenylterazine alone yielded very little background and thus exhibited excellent signal-to-background ratios , Furthermore, Diphenylterazine elicited minimal cell toxicity at millimolar concentrations. |
Animal experiment [2]: | |
Animal models |
BALB/c mice |
Preparation Method |
Bioluminescence imaging of intravenously injected HEK 293T cells:BALB/c mice are used and transfected with cells expressing biotinase gene by injecting cells into the tail vein of BALB/c mice. After the diminishing of the bioluminescence, 0.3 µmol Diphenylterazine is intraperitoneally injected. Mice are imaged with a 1-min exposure per frame over a course of 20 min. |
Dosage form |
0.3 µmol Diphenylterazine |
Applications |
Diphenylterazine was used as an emerging luciferase reporter substrate in mice. The imaging of deep tissue targets in mice was evaluated by injecting luciferase gene expressing HEK 293T cells through the tail vein. First, Diphenylterazine as a substrate has no background in control mice, and when injected into mice transfected with the luciferase gene, the fluorescence emitted by Diphenylterazine can be detected, and the intensity of Diphenylterazine has increased significantly compared with other substrates. |
References: [1]. Yeh HW, Karmach O, Ji A, Carter D, Martins-Green MM, Ai HW. Red-shifted luciferase-luciferin pairs for enhanced bioluminescence imaging. Nat Methods. 2017 Oct;14(10):971-974. doi: 10.1038/nmeth.4400. Epub 2017 Sep 4. PMID: 28869756; PMCID: PMC5678970. |
Difenilterazina, un análogo de CTZ, es un buen sustrato para la luciferasa. La difenilterazina es un agente de bioluminiscencia. La difenilterazina por sí sola produce muy poco fondo, lo que conduce a excelentes relaciones señal-ruido. Además, la difenilterazina provoca una toxicidad celular mínima a concentraciones milimolares. La difenilterazina podría sintetizarse a partir de reactivos comerciales económicos en dos pasos con rendimientos excelentes[1].
En experimentos celulares, podemos observar que Diphenylterazine tiene una relación señal-ruido más baja que otros sustratos, y Diphenylterazine causa una citotoxicidad mínima a concentraciones milimolares.
En contraste, otros sustratos probados indujeron la muerte celular dentro del rango de concentración del sustrato probado.
En el experimento con ratones, la bioluminiscencia del sitio de superficie, a una concentración de sustrato de 0.1 mM, la luminosidad del Diphenylterazine fue mayor que la del grupo control.
Lo mismo fue cierto para la imagen de objetivos de tejidos profundos, y la inyección de Diphenylterazine en ratones BALB/c no transfundidos no produjo ninguna emisión de fondo, y la fluorescencia todavía era detectable cuando se inyectaba por vía intravenosa en los ratones[2].
References:
[1].Tian X, Zhang Y, Li X, Xiong Y, Wu T, Ai HW. A luciferase prosubstrate and a red bioluminescent calcium indicator for imaging neuronal activity in mice. Nat Commun. 2022 Jul 8;13(1):3967. doi: 10.1038/s41467-022-31673-x. PMID: 35803917; PMCID: PMC9270435.
[2]. Yeh HW, et al. Red-shifted luciferase-luciferin pairs for enhanced bioluminescence imaging. Nat Methods. 2017 Oct;14(10):971-974.
Cas No. | 344940-63-2 | SDF | |
Sinónimos | DTZ | ||
Canonical SMILES | O=C1C(CC2=CC=CC=C2)=NC3=C(C4=CC=CC=C4)NC(C5=CC=CC=C5)=CN31 | ||
Formula | C25H19N3O | M.Wt | 377.44 |
Solubility | DMF : 11 mg/mL | Storage | Store at -20°C, protect from light |
General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. |
Prepare stock solution | |||
1 mg | 5 mg | 10 mg | |
1 mM | 2.6494 mL | 13.2471 mL | 26.4943 mL |
5 mM | 0.5299 mL | 2.6494 mL | 5.2989 mL |
10 mM | 0.2649 mL | 1.3247 mL | 2.6494 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
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