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Eicosapentaenoyl Ethanolamide-d4 (Synonyms: EPEAd4)

Catalog No.GC47283

An internal standard for the quantification of EPEA

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Eicosapentaenoyl Ethanolamide-d4 Chemical Structure

Cas No.: 946524-41-0

Tamaño Precio Disponibilidad Cantidad
100 μg
47,00 $
Disponible
500 μg
216,00 $
Disponible
1 mg
384,00 $
Disponible
5 mg
1.679,00 $
Disponible

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Sample solution is provided at 25 µL, 10mM.

Description of Eicosapentaenoyl Ethanolamide-d4

Eicosapentaenoyl ethanolamide (EPEA)-d4 contains four deuterium atoms at the 1, 1', 2, and 2' positions. It is intended for use as an internal standard for the quantification of EPEA by GC- or LC-mass spectrometry. Eicosapentaenoyl Ethanolamide (EPEA) is an N-acylethanolamide that inhibits dietary-restriction-induced lifespan extension in wild type and TOR pathway mutant nematodes.1 Produced endogenously from eicosapentaenoic acid, EPEA serves as a metabolic signal that couples nutrient availability with growth and lifespan. EPEA also has anti-inflammatory properties, suppressing the expression of IL-6 and MCP-1 in 3T3-L1 adipocytes in response to lipopolysaccharide.2

1.Lucanic, M., Held, J.M., Vantipalli, M.C., et al.N-acylethanolamine signalling mediates the effect of diet on lifespan in Caenorhabditis elegansNature473(7346)226-229(2011) 2.Balvers, M.G., Verhoeckx, K.C., Plastina, P., et al.Docosahexaenoic acid and eicosapentaenoic acid are converted by 3T3-L1 adipocytes to N-acyl ethanolamines with anti-inflammatory propertiesBiochimica et Biophysica Acta1801(10)1107-1114(2010)

Chemical Properties of Eicosapentaenoyl Ethanolamide-d4

Cas No. 946524-41-0 SDF
Sinónimos EPEAd4
Canonical SMILES O=C(NC([2H])([2H])C([2H])([2H])O)CCC/C=C\C/C=C\C/C=C\C/C=C\C/C=C\CC
Formula C22H31D4NO2 M.Wt 349.5
Solubility DMF: 30 mg/ml,DMSO: 30 mg/ml,Ethanol: 30 mg/ml,Ethanol:PBS (pH 7.2)(1:2): 0.3 mg/ml Storage Store at -20°C
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table of Eicosapentaenoyl Ethanolamide-d4

Prepare stock solution
1 mg 5 mg 10 mg
1 mM 2.8612 mL 14.3062 mL 28.6123 mL
5 mM 0.5722 mL 2.8612 mL 5.7225 mL
10 mM 0.2861 mL 1.4306 mL 2.8612 mL
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In vivo Formulation Calculator (Clear solution) of Eicosapentaenoyl Ethanolamide-d4

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.

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Average Rating: 5 ★★★★★ (Based on Reviews and 12 reference(s) in Google Scholar.)

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