Hygromycin B |
Catalog No.GC15496 |
La hidromicina B es un antibiótico aminoglucósido producido por Streptomyces hygroscopicus.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 31282-04-9
Sample solution is provided at 25 µL, 10mM.
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Cell experiment [1]: | |
Cell lines |
Wild type haploid Saccharomyces cerevisiae strain Y166 and the spontaneous CRY6 mutant |
Preparation Method |
Logarithmically growing yeast cells (about 2- 107/ml) were converted into spheroplasts by treatment with glusulase. The spheroplasts were recovered by incubation at 30℃ for 90 min in YEPD medium plus I M sorbitol. Then the culture was divided in three aliquots and each received 10 µCi/ml of [3H]leucine (54 Ci/mmol). One aliquot served as control, the other two received 250 and 500 µg/ml hygromycin B, respectively, and incubation was continued at 30℃. |
Reaction Conditions |
50 and 500 µg/ml,30℃,20-120 min. |
Applications |
Protein synthesis by yeast spheroplasts is blocked by hygromycin B as determined by the uptake of [3H]leucine into trichloroacetic acid-precipitable material |
References: [1]: Gonzalez A, Jimenez A, Vazquez D, Davies JE, Schindler D. Studies on the mode of action of hygromycin B, an inhibitor of translocation in eukaryotes. Biochim Biophys Acta 1978; 521:459-469. |
La hidromicina B es un antibiótico aminoglucósido producido por Streptomyces hygroscopicus. Ampliamente utilizado en medicina veterinaria y en selecciones de cultivos celulares, mata bacterias, hongos y células eucariotas superiores, incluidas las células mamíferas [1].
La sensibilidad de varias líneas celulares cultivadas al Hygromycin B fue evaluada mediante la siembra de células a baja densidad en medio Dulbecco modificado con águila suplementado con un 10% de suero fetal bovino y Hygromycin B para obtener concentraciones finales del fármaco que oscilan entre 50 y 400 ug/ml del medio. Por lo general, se producían uno o dos ciclos de replicación antes del inicio de la citotoxicidad; la muerte celular comenzaba aproximadamente tres días después del comienzo del tratamiento con el fármaco y era generalmente completa después de ocho días (las células CV1 y HeLa a veces requerían entre diez y doce días antes de que se completara la eliminación celular). Hasta ahora, no se ha encontrado ninguna línea celular naturalmente resistente al Hygromycin B [2].
El gen de resistencia a la Hygromycin B bacteriana E.coli proporciona una base para probar la utilidad de la Hygromycin B como marcador selectivo dominante en células susceptibles a la Hygromycin B. También es posible utilizar secuencias codificantes sin promotor del gen de resistencia a la Hygromycin B como sonda promotora [3].
La hidromicina B proporciona un sistema de selección generalmente aplicable para experimentos de transferencia de ADN entre células procariotas y eucariotas [2].
References:
[1]. Borovinskaya MA, Shoji S, Fredrick K, Cate JH. 2008. Structural basis for hygromycin B inhibition of protein biosynthesis. RNA 14: 1590–99
[2]. KAREN BLOCHLINGER,HEIDI DIGGELMANN. Hygromycin B Phosphotransferase as a Selectable Markerfor DNA Transfer Experiments with Higher Eucaryotic Cells. MOLECULAR AND CELLULAR BIOLOGY, Dec.1984, p.2929-2931
[3]. R. N. Rao, N. E. Allen, J. N. J. Hobbs, W. E. J. Alborn, H. A. Kirst & J. W. Paschal: Genetic and enzymatic basis of hygromycin B resistance in Escherichia coli. Antimicrob. Agents Chemother. 24, 689-695 (1983)
Cas No. | 31282-04-9 | SDF | |
Chemical Name | (3'R,3aS,4S,4'R,5'R,6R,6'R,7S,7aS)-4-[(1R,2S,3R,5S,6R)-3-amino-2,6-dihydroxy-5-(methylamino)cyclohexyl]oxy-6'-[(1S)-1-amino-2-hydroxyethyl]-6-(hydroxymethyl)spiro[4,6,7,7a-tetrahydro-3aH-[1,3]dioxolo[4,5-c]pyran-2,2'-oxane]-3',4',5',7-tetrol | ||
Canonical SMILES | CNC1CC(C(C(C1O)OC2C3C(C(C(O2)CO)O)OC4(O3)C(C(C(C(O4)C(CO)N)O)O)O)O)N | ||
Formula | C20H37N3O13 | M.Wt | 527.5 |
Solubility | ≥ 26.375mg/mL in Water | Storage | Store at -20°C |
General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. |
Prepare stock solution | |||
1 mg | 5 mg | 10 mg | |
1 mM | 1.8957 mL | 9.4787 mL | 18.9573 mL |
5 mM | 0.3791 mL | 1.8957 mL | 3.7915 mL |
10 mM | 0.1896 mL | 0.9479 mL | 1.8957 mL |
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Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Average Rating: 5
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