Maduramicin (Synonyms: Antibiotic X 14868A) |
Catalog No.GC11509 |
polyether ionophore antibiotic
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 79356-08-4
Sample solution is provided at 25 µL, 10mM.
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
IC50: 20.7 μM: inhibits ATP synthesis in spinach chloroplasts [1].
IC50: 96.6 μM: blocks H+ uptake in spinach chloroplasts [1].
Maduramicin, a natural polyether antibiotic, is cytotoxic against Cryptosporidium spp. and Plasmodium gametocytes, isolated from the actinomycete Actinomadura rubra. As a broad-spectrum anticoccidial, maduramicin, which is commonly used in veterinary medicine as an anti-coccidial agent, is active against Treponema and Cryptosporidium. Maduramicin is an ionophore which can form complexes with monovalent cations with a higher affinity for K+ than Na+.
In vitro: Maduramicin concentration-dependently blocked cell growth and inhibited cell proliferation in a concentration- and time-dependent fashion in skeletal muscle cells, mouse myoblasts (C2C12) and human rhabdomyosarcoma (RD and Rh30) cells. Also, maduramicin triggered cleavage of poly ADP ribose polymerase in a concentration-dependent manner, which was the hallmark of caspase-dependent apoptosis in C2C12 and RD cells. It was determined that maduramicin caused cell death via caspase-dependent and -independent manner in C2C12 cells [2].
In vivo: Severe combined immune deficient (SCID) mice were administered with maduramicin at 3 mg/kg of body weight per day by oral gavage for 28 days. Maduramicin treatment decreased the fecal parasite load after 21 days of treatment. Also, maduramicin treatment elicited weight loss in SCID mice. In addition, Maduramicin exerted remarkable anticryptosporidial activities with concomitant moderate toxicity on SCID mice [3].
References:
[1]. Gutiérrez-Lugo, M., Lotina-Hennsen, B., Farrés, A., Sánchez, S., & Mata, R. Phytotoxic and Photosynthetic Activities of Maduramicin and Maduramicin Methyl Ester. Zeitschrift Für Naturforschung C. 1999; 54(5-6).
[2]. Chen, X., Gu, Y., Singh, K., Shang, C., Barzegar, M., Jiang, S., & Huang, S. Maduramicin Inhibits Proliferation and Induces Apoptosis in Myoblast Cells. Plos ONE.2014; 9(12): e115652.
[3]. Mead, J., You, X., Pharr, J., Belenkaya, Y., Arrowood, M., Fallon, M., & Schinazi, R. Evaluation of maduramicin and alborixin in a SCID mouse model of chronic cryptosporidiosis. Antimicrobial Agents and Chemotherapy. 1995; 39(4): 854-858.
Cas No. | 79356-08-4 | SDF | |
Sinónimos | Antibiotic X 14868A | ||
Chemical Name | (2R,3S,4S,5R,6S)-6-[(1R)-1-[(2S,5R,7S,8R,9S)-2-[(2S,2'R,3'S,5R,5'R)-3'-[(2,6-dideoxy-3,4-di-O-methyl-β-L-arabino-hexopyranosyl)oxy]octahydro-2-methyl-5'-[(2S,3S,5R,6S)-tetrahydro-6-hydroxy-3,5,6-trimethyl-2H-pyran-2-yl][2,2'-bifuran]-5-yl]-9-hydroxy-2,8-d | ||
Canonical SMILES | CO[C@@H]1[C@@H](OC)[C@H](C)O[C@@](O[C@@H]2[C@@]([C@]3(C)CC[C@@]([C@@]4(C)O[C@@]5(C[C@H](O)[C@@H](C)[C@]([C@@H](C)[C@]6([H])O[C@@](CC(O)=O)(O)[C@@H](C)[C@H](OC)[C@H]6OC)([H])O5)CC4)([H])O3)([H])O[C@]([C@]7([H])[C@@H](C)C[C@@H](C)[C@@](C)(O)O7)([H])C2) | ||
Formula | C47H80O17 | M.Wt | 917.1 |
Solubility | DMF: soluble,DMSO: soluble,Ethanol: soluble,Methanol: soluble | Storage | Store at -20°C |
General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. |
Prepare stock solution | |||
1 mg | 5 mg | 10 mg | |
1 mM | 1.0904 mL | 5.452 mL | 10.9039 mL |
5 mM | 0.2181 mL | 1.0904 mL | 2.1808 mL |
10 mM | 0.109 mL | 0.5452 mL | 1.0904 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Average Rating: 5
(Based on Reviews and 10 reference(s) in Google Scholar.)GLPBIO products are for RESEARCH USE ONLY. Please make sure your review or question is research based.
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