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Vorinostat (SAHA, MK0683) (Synonyms: Suberoylanilide Hydroxamic Acid, Vorinostat)

Catalog No.GC17390

Un inhibidor de HDAC

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Vorinostat (SAHA, MK0683) Chemical Structure

Cas No.: 149647-78-9

Tamaño Precio Disponibilidad Cantidad
10mM (in 1mL DMSO)
41,00 $
Disponible
500mg
59,00 $
Disponible

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Sample solution is provided at 25 µL, 10mM.

Product has been cited by 1 publications

Product Documents

Quality Control & SDS

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Protocol

Cell experiment: [1]

Cell lines

Human cutaneous T-cell lymphomas (CTCL) cell lines

Preparation method

The solubility of this compound in DMSO is >10 mM. General tips for obtaining a higher concentration: Please warm the tube at 37 °C for 10 minutes and/or shake it in the ultrasonic bath for a while.Stock solution can be stored below -20°C for several months.

Reaction Conditions

IC50: 0.146 μM HH 2.062 μM HuT78 2.697 μM MJ 1.375 μM MylA 1.510 μM SeAx 72h

Applications

Vorinostat dose-dependently reduced cell proliferation with IC50 values of 0.146 μM, 2.062 μM, 2.697 μM, 1.375 μM and 1.510 μM in HH, HuT78, MJ, MylA and SeAx cells, respectively.

Animal experiment : [2]

Animal models

C57BL/6 mice bearing Eμ-myc lymphomas

Dosage form

C57BL/6 mice bearing Eμ-myc lymphomas were injected with vorinostat (200 mg/kg i.p.) and lymphoma cells were harvested after the indicated time points. The percentage of tumor cells in the lymph node of C57BL/6mice bearing Eμ-myc lymphomas treated with vorinostat was determined by FACS analysis.

Applications

Vorinostat induced a marked accumulation of Eμ-myc lymphomas displaying DNA fragmentation in vivo.

Other notes

Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal.

References:

[1] Wozniak M B, Villuendas R, Bischoff J R, et al. Vorinostat interferes with the signaling transduction pathway of T cell receptor and synergizes with PI3K inhibitors in cutaneous T-cell lymphoma. haematologica, 2010: haematol. 2009.013870.

[2] Lindemann R K, Newbold A, Whitecross K F, et al. Analysis of the apoptotic and therapeutic activities of histone deacetylase inhibitors by using a mouse model of B cell lymphoma. Proceedings of the National Academy of Sciences, 2007, 104(19): 8071-8076.

Background

Vorinostat (ácido hidroxámico suberiloanilida, SAHA) es un inhibidor de la histona desacetilasa (HDACi), que juega un papel clave en la regulación epigenética o no epigenética, induciendo el arresto del crecimiento, la diferenciación y la apoptosis de las células tumorales.[1] Vorinostat es una pequeña molécula con fórmula C14H20N2O3 y peso molecular de 264.3. El mecanismo principal de apoptosis inducida por HDACi es la activación de la vía apoptótica intrínseca. Los HDACi pueden activar esta vía al liberar citocromo c desde las mitocondrias y regular la expresión de familia Bcl-2.[2]

References:

[1] Hui-ming Z, Qian-hai D, Wei-ping C, Ru-bin L. Vorinostat, a HDAC inhibitor, showed anti-osteoarthritic activities through inhibition of iNOS and MMP expression, p38 and ERK phosphorylation and blocking NF-kB nuclear translocation. International Immunopharmacology. 2013, 17. 329-335.
[2] Norihisa U, Sayaka K, Hisanori M, Katsuhiko Y, Airo T. Requirement of p38 MAPK for a cell-death pathway triggered by vorinostat in MDA-MB-231 human breast cancer cells. Cancer Letters. 2012, 315. 112-121.

Chemical Properties

Cas No. 149647-78-9 SDF
Sinónimos Suberoylanilide Hydroxamic Acid, Vorinostat
Chemical Name N'-hydroxy-N-phenyloctanediamide
Canonical SMILES C1=CC=C(C=C1)NC(=O)CCCCCCC(=O)NO
Formula C14H20N2O3 M.Wt 264.3
Solubility ≥ 4.4 mg/mL in DMSO, <2.45 mg/mL in EtOH, <2.61 mg/mL in Water Storage Store at -20°C
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table

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1 mg 5 mg 10 mg
1 mM 3.7836 mL 18.9179 mL 37.8358 mL
5 mM 0.7567 mL 3.7836 mL 7.5672 mL
10 mM 0.3784 mL 1.8918 mL 3.7836 mL
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Average Rating: 5 ★★★★★ (Based on Reviews and 30 reference(s) in Google Scholar.)

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