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TCEP-biotin (Synonyms: Tris(2-carboxyethyl)phosphine-biotin)

Catalog No.GC40251

TCEP-biotin is biotinylated form of the reducing agent TCEP and an affinity probe for protein lysine crotonylation.

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TCEP-biotin Chemical Structure

Size Price Stock Qty
500μg
$369.00
In stock
1mg
$663.00
In stock

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Sample solution is provided at 25 µL, 10mM.

Product Documents

Quality Control & SDS

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Background

TCEP-biotin is biotinylated form of the reducing agent TCEP and an affinity probe for protein lysine crotonylation. Covalent addition of the TCEP moiety to crotonylated lysine residues results in adduct formation that allows for detection or immobilization of crotonylated proteins through interaction with the biotin ligand. It has been used in the detection and isolation of crotonylated histones in HEK293 cells.

Chemical Properties

Cas No. SDF
Synonyms Tris(2-carboxyethyl)phosphine-biotin
Formula C25H43N4O9PS M.Wt 606.7
Solubility DMF: 25 mg/ml,DMSO: 25 mg/ml,Ethanol: 25 mg/ml,PBS (pH 7.2): 0.15 mg/ml Storage Store at -20°C
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table

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1 mg 5 mg 10 mg
1 mM 1.6483 mL 8.2413 mL 16.4826 mL
5 mM 0.3297 mL 1.6483 mL 3.2965 mL
10 mM 0.1648 mL 0.8241 mL 1.6483 mL
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.

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Average Rating: 5 ★★★★★ (Based on Reviews and 38 reference(s) in Google Scholar.)

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