Tin protoporphyrin IX dichloride (Synonyms: NSC 267099, SnPPIX) |
| Catalog No.GC10800 |
Tin protoporphyrin IX dichloride (SnPPIX) is a synthetic heme analog that selectively inhibits Heme oxygenase 1 (HO-1) (Ki = 11 nM) over HO-2 (IC50 = 7.5 µM).
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 14325-05-4
Sample solution is provided at 25 µL, 10mM.
Tin protoporphyrin IX dichloride (SnPPIX) is a synthetic heme analog that selectively inhibits Heme oxygenase 1 (HO-1) (Ki = 11 nM) over HO-2 (IC50 = 7.5 µM)[1-2]. Tin protoporphyrin IX dichloride can weakly inhibit the activities of endothelial nitric oxide synthase and soluble guanylate cyclase with IC50 values of 35 and 30 μM, respectively[2]. Tin protoporphyrin IX dichloride is commonly used to study the role of HO-1 in cells and animals.
Tin protoporphyrin IX dichloride (0.5, 1, 5, 10 and 25 µM) treated 293T cells for 6 hours inhibited HO-1 activity in cells, but Tin protoporphyrin IX dichloride also upregulated HO-1 mRNA and protein levels[3]. Tin protoporphyrin IX dichloride (10 µM) could inhibit HO-1 activity and induce HO-1 protein expression in mouse fibroblasts[4]. In addition, 10 µM Tin protoporphyrin IX dichloride significantly increased HMOX1 mRNA levels in UOK 262 cells[5].
Mice infected with aerosolized Mycobacterium tuberculosis and treated with Tin protoporphyrin IX dichloride (5 mg/kg) on the same day showed a significant reduction in lung bacterial burden at week 6. Similar effects were seen when Tin protoporphyrin IX dichloride was started in week 4 of infection[6].
References:
[1] Drummond, G.S., and Kappas, A. Prevention of neonatal hyperbilirubinemia by tin protoporphyrin IX, a potent competitive inhibitor of heme oxidation. Proceedings of the National Academy of Sciences of the United States of America 78(10), 6466-6470 (1981).
[2] Zakhary, R., Gaine, S.P., Dinerman, J.L., et al. Heme oxygenase 2: Endothelial and neuronal localization and role in endothelium-dependent relaxation. Proceedings of the National Academy of Sciences of the United States of America 93(2), 795-798 (1996).
[3] Mucha O, Podkalicka P, Czarnek M, et al. Pharmacological versus genetic inhibition of heme oxygenase-1: the comparison of metalloporphyrins, shRNA and CRISPR/Cas9 system[J]. 2018.
[4] Stepniewski J, Pacholczak T, Skrzypczyk A, et al. Heme oxygenase‐1 affects generation and spontaneous cardiac differentiation of induced pluripotent stem cells[J]. IUBMB life, 2018, 70(2): 129-142.
[5] Podkalicka P, Mucha O, Kruczek S, Biela A, Andrysiak K, Stępniewski J, Mikulski M, Gałęzowski M, Sitarz K, Brzózka K, Józkowicz A, Dulak J, Łoboda A. Synthetically Lethal Interactions of Heme Oxygenase-1 and Fumarate Hydratase Genes. Biomolecules. 2020 Jan 16;10(1):143.
[6] Costa D L, Namasivayam S, Amaral E P, et al. Pharmacological inhibition of host heme oxygenase-1 suppresses Mycobacterium tuberculosis infection in vivo by a mechanism dependent on T lymphocytes[J]. MBio, 2016, 7(5): 10.1128/mbio. 01675-16.
| Cell experiment [1]: | |
Cell lines | 293T cell line |
Preparation Method | After 293T cells were treated with Tin protoporphyrin IX dichloride for 6 h or 24 h, bilirubin (HO) activity and HO-1 mRNA and protein levels were detected. |
Reaction Conditions | 0.5,1,5,10 and 25 µM,6 h or 24 h |
Applications | Tin protoporphyrin IX dichloride can inhibit Heme oxygenase (HO) activity in 293T cells after 6 h, but it can upregulate HO-1 mRNA and protein levels after 24 h. |
| Animal experiment [2]: | |
Animal models | M. tuberculosis-infected C57BL/6 (WT) mice |
Preparation Method | Mice were intraperitoneally injected with Tin protoporphyrin IX dichloride (5 mg/kg) daily on the same day of aerosol M. tuberculosis infection. Mice were euthanized at 3 and 6 weeks, and the number of lung colonies was measured. |
Dosage form | 5 mg/kg/day,3 or 6 weeks,i.p. |
Applications | At the sixth week, Tin protoporphyrin IX dichloride significantly reduced the bacterial load in the lungs. |
References: | |
| Cas No. | 14325-05-4 | SDF | |
| Synonyms | NSC 267099, SnPPIX | ||
| Chemical Name | (OC-6-13)-dichloro[7,12-diethenyl-3,8,13,17-tetramethyl-21H,23H-porphine-2,18-dipropanoato(4-)-κN21,κN22,κN23,κN24]-stannate(2-), dihydrogen | ||
| Canonical SMILES | C=CC1=C(/C2=C([H])/C(C(C)=C/3CCC([O-])=O)=NC3=C([H])/C4=N/C(C(C)=C4CCC([O-])=O)=C([H])\C5=C(C(C)=C([N-]5)/C([H])=C1\[N-]2)C=C)C.Cl.Cl.[Sn+4] | ||
| Formula | C34H32Cl2N4O4Sn | M.Wt | 750.25 |
| Solubility | 0.5 mg/ml in DMSO, 1 mg/ml in DMF,0.1 M NaOH : 14.29 mg/mL (19.05 mM; ultrasonic and adjust pH to 12 with NaOH) | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.3329 mL | 6.6644 mL | 13.3289 mL |
| 5 mM | 0.2666 mL | 1.3329 mL | 2.6658 mL |
| 10 mM | 0.1333 mL | 0.6664 mL | 1.3329 mL |
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- Purity: >95.00%
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Average Rating: 5 (Based on Reviews and 26 reference(s) in Google Scholar.)
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