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Trichostatin A (TSA)

Catalog No.GC15526

Trichostatin A (TSA) Chemical Structure

A HDAC inhibitor

Size Price Stock Qty
10mM (in 1mL DMSO)
$115.00
In stock
2mg
$69.00
In stock
5mg
$139.00
In stock
10mg
$203.00
In stock
25mg
$490.00
In stock
50mg
$840.00
In stock

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Sample solution is provided at 25 µL, 10mM.

Product Citations

Quality Control

Quality Control & SDS

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Protocol

Cell experiment[1]:

Cell lines

Human breast cancer cell line

Preparation method

The solubility of this compound in DMSO is

Reaction Conditions

10 μM TSA solved in growth medium containing 0.1% (v/v) ethanol for 96 h incubation

Applications

TSA inhibited proliferation of eight breast carcinoma cell lines with mean ± SD IC50 of 124.4 ± 120.4 nM (range, 26.4–308.1 nM). TSA treatment resulted in pronounced histone H4 hyperacetylation.

Animal experiment [1]:

Animal models

Inbred virgin female (Ludwig/Wistar/Olac) rats bearing tumors induced with NMU

Dosage form

500 μg/kg by injection daily for 4 weeks

Applications

TSA had pronounced antitumor activity in vivo. that The antitumor activity of TSA attributabled to induction of differentiation.

Other notes

Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal.

References:

1. Vigushin DM1, Ali S, Pace PE et al. Trichostatin A is a histone deacetylase inhibitor with potent antitumor activity against breast cancer in vivo. Clin Cancer Res. 2001 Apr;7(4):971-6.

Background

Trichostatin A (TSA) is a potent inhibitor of histone deacetylase (HDAC) as well as an antifungal antibiotic with cytostatic and differentiating properties that noncompetivively and reversibly inhibits HDAC, at low nanomolar concentrations, in both cultured mammalian cells and fractionated cell nuclear extracts. It is capable of arresting cells in G1 and G2 phases of the cell cycle, inducing differentiation and reverting the transformed morphology of cells in culture. According to a study investigating the effect of TSA in human breast cancer cell lines, TSA inhibited proliferation of breast carcinoma cell lines (IC50 124.4 ± 120.4 nM), comparing to all cell lines (IC50 2.4 ± 0.5 nM), and resulted in pronounced histone H4 hyperacetylation.

Reference

[1].David M. Vigushin, Simak Ali, Paul E. Pace, Nina Mirsaidi, Kazuhiro Ito, Ian Adcock, and R. Charles Coombes. Trichostatin A is a histone deacetylase inhibitor with potent antitumot activity against breast cancer in vivo. Clin Cancer Res 2001;7:971-976

Chemical Properties

Cas No. 58880-19-6 SDF
Synonyms Trichostatin A,TSA
Chemical Name (2E,4E,6R)-7-[4-(dimethylamino)phenyl]-N-hydroxy-4,6-dimethyl-7-oxohepta-2,4-dienamide
Canonical SMILES CC(C=C(C)C=CC(=O)NO)C(=O)C1=CC=C(C=C1)N(C)C
Formula C17H22N2O3 M.Wt 302.37
Solubility ≥ 15.12mg/mL in DMSO, ≥ 16.56 mg/mL in EtOH with ultrasonic Storage Desiccate at -20°C
General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.
Shipping Condition Evaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
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3. All of the above co-solvents are available for purchase on the GlpBio website.

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Average Rating: 5 ★★★★★ (Based on Reviews and 35 reference(s) in Google Scholar.)

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