tumor protein p53 binding protein fragment [Homo sapiens]/[Mus musculus]
Sample solution is provided at 25 µL, 10mM.
GlpBio Products Cited In Reputable Papers
P53 is a sequence-specific DNA-binding oligomeric protein that can activate transcription from promoters bearing p53-binding sites. Whereas the activation region of p53 has been identified within the amino terminus, the location of the specific DNA-binding domain has not been reported1.
Tumor protein p53 binding protein (53BP1) has been identified in a yeast two hybrid screen as a protein that interacts with the central DNA-binding domain of p532. Similar to breast cancer susceptibility gene 13, 4 (BRCA1; 53BP1 enhances p53-dependent transcription5. Interestingly, the COOH terminus of 53BP1 contains tandem BRCA1 COOH terminus (BRCT) motifs.
53BP1 becomes hyperphosphorylated and rapidly relocates to the sites of DNA strand breaks in response to ionizing radiation. 53BP1 foci formation is reduced in ATM-deficient cells and can be inhibited by wortmannin in ATM wild-type cells. Moreover, radiation-induced hyperphosphorylation of 53BP1 is absent in cells treated with wortmannin, as well as in ATM-deficient cells. 53BP1 participates in DNA damage-signaling pathways and is regulated by ATM after ǃ-radiation6.
1.J.Bargonetti, J. J. Manfredi et al. A proteolytic fragment from the central region of p53 has marked sequence-specific DNA-binding activity wlien generated from wild-type but not from oncogenic mutant p53 protein, Genes Dev. 1993 7: 2565-2574
2.Iwabuchi, K., P.L. Bartel, B. Li, R. Marraccino, S. Fields (1994) Two cellular proteins that bind to wild-type but not mutant p53. Proc. Natl. Acad. Sci. USA91:6098-6102
3.Ouchi, T., A.N. Monteiro, A. August, S.A. Aaronson, H. Hanafusa (1998) BRCA1 regulates p53-dependent gene expression. Proc. Natl. Acad. Sci. USA95:2302-2306
4.Zhang, H., K. Somasundaram, Y. Peng, H. Tian, D. Bi, B.L. Weber, W.S. El-Deiry BRCA1 physically associates with p53 and stimulates its transcriptional activity Oncogene16199817131721
5.Iwabuchi, K., B. Li, H.F. Massa, B.J. Trask, T. Date, S. Fields(1998) Stimulation of p53-mediated transcriptional activation by the p53-binding proteins, 53BP1 and 53BP2. J. Biol. Chem 273:26061-2606
6.Irene Rappold, Kuniyoshi Iwabuchi, Takayasu Date, and Junjie Chen Tumor Suppressor P53 Binding Protein 1 (53bp1) Is Involved in DNA Damage-Signaling Pathways. JCB vol. 153 no. 3 613-620
|Solubility||≥ 124.2mg/mL in DMSO||Storage||Store at -20°C|
|General tips||For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.|
|Shipping Condition||Evaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request
In vivo Formulation Calculator (Clear solution)
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Average Rating: 5(Based on Reviews and 28 reference(s) in Google Scholar.)
GLPBIO products are for RESEARCH USE ONLY. Please make sure your review or question is research based.
Required fields are marked with *