vitamin D binding protein precursor (353-363) [Homo sapiens] (Synonyms: H2N-Arg-Thr-His-Leu-Pro-Glu-Val-Phe-Leu-Ser-Lys-OH ) |
| Catalog No.GP10001 |
Highly polymorphic serum glycoprotein
Products are for research use only. Not for human use. We do not sell to patients.
![vitamin D binding protein precursor (353-363) [Homo sapiens] Chemical Structure](/media/struct/GP1/GP10001.png "vitamin D binding protein precursor (353-363) [Homo sapiens] Chemical Structure")
Sample solution is provided at 25 µL, 10mM.
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Human vitamin D-binding protein (hDBP), also known as Gcglobulin, is an abundant, multifunctional, and highly polymorphic serum glycoprotein synthesized by the liver1. DBP is the major serum transport protein for the vitamin D sterols2, binds and sequesters monomers of actin with high affinity3, 4, and has been identified on the surface of a variety of cell types including B-lymphocytes5, subpopulations of T-lymphocytes6, and the cytotrophoblasts of the placenta7.
DBP is a secreted protein and would therefore be predicted to have a signal sequence. The amino-terminus of the mature serum protein was established by aligning the sequence predicted by the cDNA to a sequenced amino-terminal hDBP peptide8.
References:
1.Haddad, J. G. 1979. Transport of vitamin D metabolites. Clin. Orthop. Relat. Res. 142:249-274.
2.Daiger, S. P., M. S. Schanfield, and L. L. Cavalli-Sforza. 1975. Group specific component (Gc) proteins bind vitamin D and 25-hydroxyvitamin D. Proc. Natl. Acad. Sci. USA. 72:2076-2080.
3.Cooke, N. E., J. Walgate, and J. G. Haddad. 1979. Human serum binding protein for vitamin D and its metabolites. I. Physicochemical and immunological identification in human tissues. J. Biol. Chem. 254: 5965-5971.
4.Van Baelen, H., R. Bouillon, and P. DeMoor. 1980. Vitamin D binding protein (Gc-Globulin) binds actin. J. Biol. Chem. 255:2270-2272.
5.Petrini, M., D. L. Emerson, and R. M. Galbraith. 1983. Linkage between surface immunoglobulin and cytoskeleton of B lymphocytes may involve Gc protein. Nature (Lond.). 306:73-74.
6.Petrini, M., R. M. Galbraith, D. L. Emerson, A. E. Nel, and P. Arnaud. 1985. Structural studies of T lymphocyte Fc receptors. J. Biol. Chem. 260:1804-1810.
7.Nestler, J. E., E. Lange, J. McLeod, H. J. Kliman, and J. F. Strauss. 1985. Detection of vitamin D binding protein (DBP) in human cytotrophoblasts. Seventh Annual American Societyfor Bone and Mineral Research. Wash. DC. 266a. (Abstr.).
8.Svasti, J., A. Kurosky, A. Bennett, and B. H. Bowman. 1979. Molecular basis for the three major forms of human serum vitamin D binding protein (group specific component). Biochemistry. 18:1611-1617.
| Cas No. | SDF | ||
| Synonyms | H2N-Arg-Thr-His-Leu-Pro-Glu-Val-Phe-Leu-Ser-Lys-OH | ||
| Canonical SMILES | NC(CCCNC(N)=N)C(NC(C(C)O)C(NC(CC1=CN=CN1)C(NC(CC(C)C)C(N(CCC2)C2C(NC(CCC(O)=O)C(NC(C(C)C)C(NC(CC3=CC=CC=C3)C(NC(CC(C)C)C(NC(CO)C(NC(CCCCN)C(O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O | ||
| Formula | C61H99N17O16 | M.Wt | 1326.54 |
| Solubility | ≥132.7mg/mL in DMSO | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 0.7538 mL | 3.7692 mL | 7.5384 mL |
| 5 mM | 0.1508 mL | 0.7538 mL | 1.5077 mL |
| 10 mM | 0.0754 mL | 0.3769 mL | 0.7538 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)
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