DTLMISR acetate

DTLMISR acetate is a tryptic peptide widely present in the Fc regions of all four human IgG subclasses. DTLMISR acetate contains a methionine residue susceptible to both post-translational modification oxidation and artificial oxidation, and its oxidized form requires specific detection via LC-MRM before SILuMAb aliquoting and storage ^{[1] [2] [3] [4]}.

References:
[1]. Lesur A, Varesio E, Hopfgartner G. Protein quantification by MALDI-selected reaction monitoring mass spectrometry using sulfonate derivatized peptides. Analytical chemistry. 2010 Jun 15;82(12):5227-37.
[2]. Vialaret J, Broutin S, Pugnier C, et al.. What sample preparation should be chosen for targeted MS monoclonal antibody quantification in human serum?. Bioanalysis. 2018 May 01;10(10):723-735.
[3]. Hong Q, Lebrilla CB, Miyamoto S, et al.. Absolute quantitation of immunoglobulin G and its glycoforms using multiple reaction monitoring. Analytical chemistry. 2013 Sep 17;85(18):8585-93.
[4]. Kleinberg A, Yoon J, Mao Y, et al.. Eliminating liquid chromatography-induced methionine oxidation of monoclonal antibodies using high-concentration TCEP injections: The "anTCEPtic" column cleaning method. Journal of pharmaceutical sciences. 2026 Jun;115(6):104298.