GLPBio

Fmoc-Arg-OH.HCl

Catalog No.: GA10558Purity:>99.50%
Fmoc-Arg-OH.HCl is an Fmoc-protected arginine derivative.

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Fmoc-Arg-OH.HCl
Cas No.: 131669-11-9
25gIn Stock
$196.00
1
100gIn Stock
$580.00
1

Citations

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    Nature
    641, 529–536 (2025)
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    Nature
    628, 630–638 (2024)
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    Nature
    632, 686–694 (2024)
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    Nature
    618, 1017–1023 (2023)
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    Nature
    610, 366–372 (2022)
  • Cell cover
    Cell
    187(9):2288-2304 (2024)
  • Cell cover
    Cell
    183(7):1867-1883 (2020)
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    Science
    388(6745) (2025)
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    387(6739) (2025)
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    Science
    387(6734) (2025)
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    Cell Research
    35, 97–116 (2025)
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    34, 683–706 (2024)
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    33, 273–287 (2023)
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    33, 546–561 (2023)
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    33, 904–922 (2023)
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    31, 1291–1307 (2021)

Description

Fmoc-Arg-OH.HCl is an Fmoc-protected arginine derivative. Fmoc-Arg-OH.HCl is commonly used as a building block in peptide synthesis, especially in solid-phase peptide synthesis (SPPS), for the preparation of peptides, peptide analogs, and related conjugates containing the Arg sequence[1][2].

References:
[1] Fantoni T, Orlandin A, Di Stefano I, et al. Solid phase peptide synthesis using side-chain unprotected arginine and histidine with Oxyma Pure/TBEC in green solvents. Green Chem. 2024;26(21):10929-10939.
[2] Alhassan, Mahama et al. “Revisiting NO2 as Protecting Group of Arginine in Solid-Phase Peptide Synthesis.” International journal of molecular sciences vol. 21,12 4464. 23 Jun. 2020.

Protocole (Protocol)

This plan is based on literature, please modify it to meet your specific needs.
SPPS of linear vasopressin[1]
Linear vasopressin syntheses were performed in glass syringes equipped with a polyethylene porous disc and attached to a vacuum manifold for the removal of excess reagents and solvents.
1. Add 0.3g of pre-loaded Fmoc-Gly-Rink amide resin (loading capacity of 0.5mmol/g) into 3mL of NBP/DMC (8/2; v/v) mixed solvent. After standing and swelling, filter to remove the solvent.
2. Add 1.5mL of NBP/DMC containing 20% piperidine (PIP) or diethanolamine (DEAPA) to the swollen resin and shake and invert at room temperature for 15min (2 times).
3. Wash the resin with 1.5mL of NBP/DMC (3 times; 2min each).
4. Pre-activate Fmoc-Arg-OH.HCl and other Fmoc-amino acid derivatives (2 equivalents; 0.3mmol) with Oxyma Pure (2-5 equivalents; 0.3-0.75mmol) and DIC (2 equivalents; 46.4μL; 0.3mmol) for 5min, then couple with the resin. After each step of coupling, treat the peptide resin with 1.5mL of NBP/DMC containing 20% piperidine (PIP) or diethanolamine (DEAPA) (2 times; 15min each), and then wash with 1.5mL of NBP/DMC (3 times; 2min each). 
5. After Fmoc cleavage at the N-terminal amino group, further wash the peptide resin with 1.5mL of DCM (3 times; 2min each) under vacuum and dry for 12h.
6. Dissolve the dried peptide resin in 3mL of TFA/TIS/H₂O (95/2.5/2.5; v/v/v) mixed solution and stir for 2h. Then filter the resin, wash with 1mL of TFA for 1min.
7. Slowly add 10mL of cooled to 4°C diisopropyl ether to the system, filter the peptide product and vacuum dry to obtain crude linear vasopressin, which is directly analyzed by HPLC-MS.

References:
[1] Fantoni T, Orlandin A, Di Stefano I, et al. Solid phase peptide synthesis using side-chain unprotected arginine and histidine with Oxyma Pure/TBEC in green solvents. Green Chem. 2024;26(21):10929-10939.

Documents produit (Product Documents)

Purity:>99.50%Appearance:A solid

Propriétés chimiques (Chemical Properties)

N° CAS
131669-11-9
Nom chimique
(S,E)-2-((((9H-fluoren-9-yl)methoxy)(hydroxy)methylene)amino)-5-guanidinopentanoic acid hydrochloride
SMILES
N=C(NCCC[C@@](/N=C(OCC1C2=CC=CC=C2C3=CC=CC=C31)\O)([H])C(O)=O)N.Cl
Formule moléculaire
C21H24N4O4.HCl
Masse moléculaire
432.9
Solubilité
Soluble in water or 1% acetic acid
Conservation
Desiccate at -20°C
Conseils généraux
Choisissez un solvant adapté selon les données de solubilité du produit. Après préparation, répartissez la solution mère en aliquotes afin d’éviter les cycles répétés de congélation-décongélation.
Utilisez les solutions mères dans les 6 mois si elles sont conservées à -80°C, ou dans le mois si elles sont conservées à -20°C.
Pour améliorer la solubilité, réchauffez le tube à 37°C et soniquez-le pendant une durée appropriée.
Conditions d’expédition
Les échantillons d’évaluation sont expédiés avec des blocs réfrigérants. Les autres formats sont expédiés à température ambiante ou, sur demande, avec des blocs réfrigérants.

Calculateurs (Calculators)Molarité / Dilution / Masse moléculaire / Conversion d’unités / Formulation in vivo / Solubilité

g/mol

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