Citations
1 publicationsPublications de chercheurs utilisant ce produitDescription
Hepatocyte Growth Factor Human Recombinant produced in Baculovirus is a heterodimer, non-glycosylated, polypeptide chain containing 692 a.a and having a total molecular mass of 78.0 KDa.The HGF is purified by proprietary chromatographic techniques.
Documents produit (Product Documents)
Purity:> 95% as analyzed by SDS-PAGEAppearance:Sterile Filtered White lyophilized (freeze-dried) powder.
Propriétés chimiques (Chemical Properties)
Pureté
> 95% as analyzed by SDS-PAGE
Source
Escherichia Coli.
Aspect physique
Sterile Filtered White lyophilized (freeze-dried) powder.
Conditions d’expédition
Shipping with dry ice.
Synonymes
Scatter Factor (SF); Hepatopoietin (HPTA); HGF; HGFB; F-TCF.
Séquence d’acides aminés
QRKRRNTIHEFKKSAKTTLIKIDPALKIKTKKVNTADQCANRCTRNKGLPFTCKAFVFDKARKQCLWFPFNSMSSGVKKEFGHEFDLYENKDYIRNCIIGKGRSYKGTVSITKSGIKCQPWSSMIPHEHSYRGKDLQENYCRNPRGEEGGPWCFTSNPEVRYEVCDIPQCSEVECMTCNGESYRGLMDHTESGKICQRWDHQTPHRHKFLPERYPDKGFDDNYCRNPDGQPRPWCYTLDPHTRWEYCAIKTCADNTMNDTDVPLETTECIQGQGEGYRGTVNTIWNGIPCQRWDSQYPHEHDMTPENFKCKDLRENYCRNPDGSESPWCFTTDPNIRVGYCSQIPNCDMSHGQDCYRGNGKNYMGNLSQTRSGLTCSMWDKNMEDLHRHIFWEPDASKLNENYCRNPDDDAHGPWCYTGNPLIPWDYCPISRCEGDTTPTIVNLDHPVISCAKTKQLRVVNGIPTRTNIGWMVSLRYRNKHICGGSLIKESWVLTARQCFPSRDLKDYEAWLGIHDVHGRGDEKCKQVLNVSQLVYGPEGSDLVLMKLARPAVLDDFVSTIDLPNYGCTIPEKTSCSVYGWGYTGLINYDGLLRVAHLYIMGNEKCSQHHRGKVTLNESEICAGAEKIGSGPCEGDYGGPLVCEQHKMRMVLGVIVPGRGCAIPNRPGIFVRVAYYAKWIHKIILTYKVPQS.
Solubilité
It is recommended to reconstitute the lyophilized Epidermal Growth Factor in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Masse moléculaire apparente
88~90 kDa (single chain), 59~61 kDa (alpha chain), 30~34 kDa (beta chain), on SDS-PAGE under reducing conditions. Da
Stabilité
Upon receiving, this product remains stable for 6 months at lower than -70°C. Upon reconstitution, the product should be stable for 1 week at 4°C or for 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.
Activité biologique
ED50 < 10.0 ng/ml, measured in a cell proliferation assay using 4MBr5 cells, corresponding to a specific activity of > 1.0 × 105 U/mg.
Formulation
Lyophilized after extensive dialysis against PBS.
Reviews
Review for HGF Human
Rating: 5
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