INF7TAT acetate is an amphipathic cell-penetrating peptide that mediates intracellular delivery of CRISPR ribonucleoproteins. INF7TAT acetate achieves endosomal escape via the INF7 fragment and cell binding via the TAT fragment. It enables intracellular delivery into primary human T cells and hematopoietic stem and progenitor cells, facilitating gene knockout and AAV-mediated homology-directed repair. INF7TAT acetate can be used for genome editing-related research [1] [2].
In Vitro, INF7TAT acetate is the amphipathic peptide backbone of the PERC delivery system, and its engineered variants INF7TAT‑A5K / INF7TAT‑P55 enable efficient intracellular delivery of Cas9 RNP and Cas12a RNP into human primary T cells and human primary hematopoietic stem and progenitor cells (HSPC) without electroporation equipment[1].
References:
[1]. Vo N, et al. Calcium Shock Enables Efficient and Programmable Particle Delivery for Genome Editing Applications. Advanced science (Weinheim, Baden-Wurttemberg, Germany). 2026 Apr;13(21):e10441.
[2]. Sahu SU, et al. Peptide-enabled ribonucleoprotein delivery for CRISPR engineering (PERC) in primary human immune cells and hematopoietic stem cells. Nature protocols. 2025 Oct;20(10):2735-2770.
In Vitro, INF7TAT acetate is the amphipathic peptide backbone of the PERC delivery system, and its engineered variants INF7TAT‑A5K / INF7TAT‑P55 enable efficient intracellular delivery of Cas9 RNP and Cas12a RNP into human primary T cells and human primary hematopoietic stem and progenitor cells (HSPC) without electroporation equipment[1].
References:
[1]. Vo N, et al. Calcium Shock Enables Efficient and Programmable Particle Delivery for Genome Editing Applications. Advanced science (Weinheim, Baden-Wurttemberg, Germany). 2026 Apr;13(21):e10441.
[2]. Sahu SU, et al. Peptide-enabled ribonucleoprotein delivery for CRISPR engineering (PERC) in primary human immune cells and hematopoietic stem cells. Nature protocols. 2025 Oct;20(10):2735-2770.