Abz-Val-Ala-Asp-Nva-Arg-Asp-Arg-Gln-EDDnp (trifluoroacetate salt) |
カタログ番号GC42684
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Abz-Val-Ala-Asp-Nva-Arg-Asp-Arg-Gln-EDDnp (trifluoroacetate salt)は蛍光共鳴エネルギー移動（FRET）基を含有する修飾ペプチドである。
Products are for research use only. Not for human use. We do not sell to patients.
Sample solution is provided at 25 µL, 10mM.
Abz-Val-Ala-Asp-Nva-Arg-Asp-Arg-Gln-EDDnp (trifluoroacetate salt)は蛍光共鳴エネルギー移動（FRET）基を含有する修飾ペプチドである。320nmの励起波長と420nmの発光波長で蛍光シグナルをモニタリングでき、Abz-Val-Ala-Asp-Nva-Arg-Asp-Arg-Gln-EDDnpは、プロテアーゼ活性を検出する蛍光基質としてよく使われる[1]。
References:
[1] Yu J, Fu Y, Gao J, et al. Cathepsin C from extracellular histone-induced M1 alveolar macrophages promotes NETosis during lung ischemia-reperfusion injury. Redox Biol. 2024 Aug;74:103231.
細胞におけるプロテアソーム活性の測定
このプロトコールは研究データから整理されたものであり、参考までです。特定の実験需要に基づいて調整が必要な場合があります。
（1）試薬の調製：Abz-Val-Ala-Asp-Nva-Arg-Asp-Arg-Gln-EDDnp凍結乾燥粉末をジメチルホルムアミド（DMF）で溶解し、高濃度の貯蔵液（例えば5mM）を調製し、アリコートして、-20°Cで保存する。
（2）酵素サンプルの調製：精製のPR3酵素（濃度範囲は通常0.1nMから10nM）またはPR3を含有する細胞懸濁液（例えば活性化された好中球）を使う。
（3）PR3酵素活性の検出：反応混合物には20μMのAbz-Val-Ala-Asp-Nva-Arg-Asp-Arg-Gln-EDDnp、50mMのHEPES、150mMのNaClと0.05%のTriton X-100が含まれており、総体積は200μLである。37℃で30分間インキュベートする。マイクロプレート分光蛍光光度計で蛍光をモニタリングする。Abzに対する励起波長は320nmであり、発光波長は420nmである。
注意事項：
（1）遊離の蛍光基質には消光問題がある。蛍光消光を遅らせるためにはできる限り光への暴露を避けて下さい。
（2）DMFは酵素の活性を影響するかもしれない。貯蔵液におけるDMFの最終濃度をできるだけ低い水準に維持して下さい（通常は<1%）。
（3）ご自分の安全と健康の為に、実験の間には白衣と使い捨ての手袋をご着用下さい。
References:
[1] Korkmaz B, Attucci S, Epinette C, et al. Measurement of neutrophil elastase, proteinase 3, and cathepsin G activities using intramolecularly quenched fluorogenic substrates. Methods Mol Biol. 2012;844:125-38.
| Cas No. | SDF | ||
| Canonical SMILES | O=C(NCCNC1=CC=C([N+]([O-])=O)C=C1[N+]([O-])=O)[C@@H](NC([C@@H](NC([C@@H](NC([C@@H](NC([C@H](CCC)NC([C@@H](NC([C@@H](NC([C@@H](NC(C2=CC=CC=C2N)=O)C(C)C)=O)C)=O)CC(O)=O)=O)=O)CCCNC(N)=N)=O)CC(O)=O)=O)CCCNC(N)=N)=O)CCC(N)=O.FC(F)(C(O)=O)F | ||
| Formula | C53H80N20O18•XCF3COOH | M.Wt | 1285.3 |
| 溶解度 | DMF:Water: Soluble,Water: Soluble | Storage | Store at -20°C; protect from light |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 778 μL | 3.8901 mL | 7.7803 mL |
| 5 mM | 155.6 μL | 778 μL | 1.5561 mL |
| 10 mM | 77.8 μL | 389 μL | 778 μL |
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Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.50% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 33 reference(s) in Google Scholar.)