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APC-Cy7

カタログ番号GC80394 Copy One-Click Copy Product Info

APC-Cy7 is an APC-tandem fluorochrome (allophycocyanin-cyanine 7) and degradable fluorochrome, with maximum absorption at ~650 nm, peak emission at 767 nm when excited by a 633 nm red laser, and cell-dependent degradation.

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APC-Cy7 化学構造

サイズ 価格 在庫数 個数
1mg
$252.00
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Sample solution is provided at 25 µL, 10mM.



Description of APC-Cy7

APC-Cy7 is an APC-tandem fluorochrome (allophycocyanin-cyanine 7) and degradable fluorochrome, with maximum absorption at ~650 nm, peak emission at 767 nm when excited by a 633 nm red laser, and cell-dependent degradation. APC-Cy7 undergoes time-dependent degradation (decoupling) when bound to peripheral blood leukocytes, leading to nonspecific APC channel signal, with degradation cell type-dependent. APC-Cy7 shows reduced degradation when immunolabeled cells have inhibited metabolic activity or are incubated with vitamin C. APC-Cy7 serves as a tandem fluorochrome in multiparametric flow cytometry for detection of leukocyte surface proteins when conjugated to specific antibodies (Ex/Em = 650 nm/778 nm) [1].

In Vitro, APC-Cy7 (0-60 min) conjugated to anti-CD45 antibody undergoes time-dependent degradation (decoupling) in human peripheral blood lymphocytes, monocytes, and polymorphonuclear leukocytes, with lymphocyte decoupling increasing from ~5% at 0 min to ~19% at 30 min, while no decoupling occurs on antibody-bound CompBeads unless incubated with unlabeled cells[1].

References:
[1]. Le Roy C, et al. Flow cytometry APC-tandem dyes are degraded through a cell-dependent mechanism. Cytometry A. 2009;75(10):882-890.

Chemical Properties of APC-Cy7

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溶解度 Storage Store at 4°C, protect from light
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.
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