BGN3 is a good substrate for the SNAP-tag and H5 enzymes. The activities of SNAP-tag and H5 enzymes on BGN3 are reasonable (IC50= 15.6 and 23.5 μM, respectively). BGN3 is a click chemistry reagent, it contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. Strain-promoted alkyne-azide cycloaddition (SPAAC) can also occur with molecules containing DBCO or BCN groups.
Substrate specificity of SNAP-tag® and H5 by competitive inhibition method (IC50) by using SVG as substrate[1].
References:
[1]. Merlo R, et al. The SNAP-tag technology revised: an effective chemo-enzymatic approach by using a universal azide-based substrate. J Enzyme Inhib Med Chem. 2021;36(1):85-97.