Choline |
カタログ番号GB57726
One-Click Copy Product Info
|
Choline is an essential nutrient for neurotransmitter synthesis, cell-membrane signaling, lipid transport, and methyl-group metabolism.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 62-49-7
Sample solution is provided at 25 µL, 10mM.
_1-3.$$$39978408@51.jpg');)
_1-9.$$$38538795@65.jpg');)
_1-9.$$$39112701@51.jpg');)
_1-7.$$$37316672@70.jpg');)
_366-372.$$$36198801@70.jpg');)
.$$$38565142@65.jpg');)
_1867-1883.$$$33248023@67.jpg');)
_eads6055.$$$39977546@45.jpg');)
_eadm9805.$$$40080571@45.jpg');)
_eadj3020.$$$39666821@45.jpg');)
_1-20.$$$39757301@28.jpg');)
_1-24.$$$38898113@28.jpg');)
_273-287.$$$36806353@46.jpg');)
_1-16.$$$37156877@44.jpg');)
_1-19.$$$37460805@44.jpg');)
_1291-1307.$$$34518654@46.jpg');)
Choline is an essential nutrient for neurotransmitter synthesis, cell-membrane signaling, lipid transport, and methyl-group metabolism [1]. Choline can affect DNA methylation, leading to impaired DNA repair function, and regulate cell signaling mediated by phospholipid metabolic intermediates to support the synthesis of cell membranes, thereby promoting cell proliferation [2]. Choline has been widely used to promote brain development and the function of the nervous system [3].
In vitro, Choline (70μM) treatment for 2 days significantly increased the viability of PC12 cells and reduced DNA fragmentation [4]. Treatment with 13.2μM Choline for 2 hours significantly inhibited the phagocytic and killing capacity of bovine neutrophils, and increased the mRNA abundance of muscarinic and nicotinic cholinergic receptors [5]. Treatment with 70μM Choline for 72 hours significantly inhibited the accumulation of triglycerides in C3A cells, protected mitochondrial membrane potential from decrease, and increased the activity of GSH-Px in the cells [6].
In vivo, Choline treatment via oral administration at a dose of 1mg/kg/day for 14 days significantly reduced oxidative stress and inhibited inflammatory responses in a mouse model of allergic airway disease[7]. Oral administration of 4.95g/kg of Choline daily for 10 months significantly improved the cognitive deficits and anxiety symptoms in APP/PS1 mice, and inhibited the deposition of β-amyloid protein[8].
References:
[1] Zeisel S H, Da Costa K A. Choline: an essential nutrient for public health[J]. Nutrition reviews, 2009, 67(11): 615-623.
[2] Awwad H M, Geisel J, Obeid R. The role of choline in prostate cancer[J]. Clinical biochemistry, 2012, 45(18): 1548-1553.
[3] Zeisel S H. Choline: an essential nutrient for humans[J]. Nutrition, 2000, 16(7-8): 669-671.
[4] Holmes-McNary M Q, Loy R, Mar M H, et al. Apoptosis is induced by choline deficiency in fetal brain and in PC12 cells[J]. Developmental Brain Research, 1997, 101(1-2): 9-16.
[5] Garcia M, Mamedova L K, Barton B, et al. Choline regulates the function of bovine immune cells and alters the mRNA abundance of enzymes and receptors involved in its metabolism in vitro[J]. Frontiers in immunology, 2018, 9: 2448.
[6] Zhu J, Wu Y, Tang Q, et al. The effects of choline on hepatic lipid metabolism, mitochondrial function and antioxidative status in human hepatic C3A cells exposed to excessive energy substrates[J]. Nutrients, 2014, 6(7): 2552-2571.
[7] Mehta A K, Arora N, Gaur S N, et al. Choline supplementation reduces oxidative stress in mouse model of allergic airway disease[J]. European journal of clinical investigation, 2009, 39(10): 934-941.
[8] Wang Y, Guan X, Chen X, et al. Choline supplementation ameliorates behavioral deficits and Alzheimer's disease‐like pathology in transgenic APP/PS1 mice[J]. Molecular nutrition & food research, 2019, 63(18): 1801407.
| Cell experiment [1]: | |
Cell lines | PC12 cells |
Preparation Method | PC12 cells were plated on 100mm dishes at a density of 2×105 cells/cm2 in choline-free DMEM/F12 (1:1) growth media containing 5% (v/v) dialyzed horse serum and 10% (v/v) delipidated fetal calf serum for 3 days. PC12 cells were maintained, at 37°C in a humidified atmosphere, containing 5% CO2/95% air, for 2 days in serum-free DMEM/F12 media-containing 70μM Choline. The cell viability was evaluated. |
Reaction Conditions | 70μM; 2 days |
Applications | Choline treatment significantly enhanced cell viability of PC12 cells. |
| Animal experiment [2]: | |
Animal models | APP/PS1 transgenic mice |
Preparation Method | APP/PS1 transgenic mice (2 months old) were housed singly in a standard environment with water ad libitum. From 2 months to 11 months of age, mice were randomly divided into two groups: a control AIN76A diet group and a Choline-supplemented diet group (4.95g/kg/day). Then, the mouse brain tissues were collected for analysis. |
Dosage form | 4.95g/kg/day for 10 months; p.o. |
Applications | Choline supplementation decreased Aβ deposition and inhibited microgliosis in APP/PS1 mice. |
References: | |
| Cas No. | 62-49-7 | SDF | |
| Formula | C5H14NO+ | M.Wt | 104.17 |
| 溶解度 | ≥50mg/ml in DMSO; ≥50mg/ml in water | Storage | Store at 2-8°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 9.5997 mL | 47.9985 mL | 95.9969 mL |
| 5 mM | 1.9199 mL | 9.5997 mL | 19.1994 mL |
| 10 mM | 960 μL | 4.7998 mL | 9.5997 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >90.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)