Concanavalin A -HRP (Con A-HRP) is a HRP-labled Concanavalin A. Concanavalin A is a Ca 2+ /Mn 2+ -dependent and mannose/glucose-binding plant lectin. Concanavalin A binds the N- and O-glycosylated proteins of head and neck carcinoma cells glycoproteins [1] [2].
In Vitro, Concanavalin A-HRP (1:100; 2 h) specifically binds and visualizes glycoproteins with α-D-mannopyranosyl or high-mannose carbohydrate chains (including transferrin, bovine IgG, α1-antitrypsin, and ovalbumin) on Western blots[1]. Concanavalin A-HRP labels specific intracellular structures in human pituitary adenoma cells, including nuclear envelope, endoplasmic reticulum, Golgi apparatus, plasma membrane, and immature/small secretory granules in select subtypes[2]. Concanavalin A-HRP labels secretory granules in oncocytic human pituitary non-functioning adenoma cells[2]. Concanavalin A-HRP acts as an electron microscopic staining agent to detect saccharide residues in biological structures and distinguish subtypes of human pituitary adenomas based on binding patterns[2].
References:
[1]. Eric E. Snyder, et al. Western Blotting with a Concanavalin A-Horseradish Peroxidase Conjugate. Biochemical Education. 1990. Vol. 18, No. 3, pp. 147-148.
[2]. Hori T, et al. Localization of concanavalin A binding sites in human pituitary adenoma cells as revealed by HRP-labelling method. Acta Neuropathol. 1983;62(1-2):59-66.
In Vitro, Concanavalin A-HRP (1:100; 2 h) specifically binds and visualizes glycoproteins with α-D-mannopyranosyl or high-mannose carbohydrate chains (including transferrin, bovine IgG, α1-antitrypsin, and ovalbumin) on Western blots[1]. Concanavalin A-HRP labels specific intracellular structures in human pituitary adenoma cells, including nuclear envelope, endoplasmic reticulum, Golgi apparatus, plasma membrane, and immature/small secretory granules in select subtypes[2]. Concanavalin A-HRP labels secretory granules in oncocytic human pituitary non-functioning adenoma cells[2]. Concanavalin A-HRP acts as an electron microscopic staining agent to detect saccharide residues in biological structures and distinguish subtypes of human pituitary adenomas based on binding patterns[2].
References:
[1]. Eric E. Snyder, et al. Western Blotting with a Concanavalin A-Horseradish Peroxidase Conjugate. Biochemical Education. 1990. Vol. 18, No. 3, pp. 147-148.
[2]. Hori T, et al. Localization of concanavalin A binding sites in human pituitary adenoma cells as revealed by HRP-labelling method. Acta Neuropathol. 1983;62(1-2):59-66.