D-Xylulose 5-phosphate sodium is a pentose phosphate ester and an essential intermediate metabolite in the pentose phosphate pathway (PPP) and the non-oxidative phase of the pentose phosphate pathway. D-xylulose-5-phosphate sodium can be efficiently synthesized through the phosphorylation of D-xylulose catalyzed by D-xylulokinase (XKS1 from Saccharomyces cerevisiae ), with ATP regeneration facilitated by the phosphoenolpyruvate (PEP)/pyruvate kinase (PK) system [1].
In Vitro, D-Xylulose 5-phosphate sodium (up to 500 mM; 6 h) is stable at low pH but unstable under alkaline conditions at 25 °C, with degradation increasing with higher concentrations over 6 h[2]. D-Xylulose 5-phosphate sodium (5-100 mM GA3P or DHAP) is enzymatically synthesized from 5-100 mM GA3P or DHAP using cTK and TPI at 25 °C, with yield decreasing and initial production rate peaking at 80 mM as substrate concentration increases[2].
References:
[1]. Norman Hardt, et al. Facile synthesis of D-xylulose-5-phosphate and L-xylulose-5-phosphate by xylulokinase-catalyzed phosphorylation. 22 Jun 2019.
[2]. Shaeri J, et al. Characterization of enzymatic D-xylulose 5-phosphate synthesis. Biotechnol Bioeng. 2008;101(4):761-767.
In Vitro, D-Xylulose 5-phosphate sodium (up to 500 mM; 6 h) is stable at low pH but unstable under alkaline conditions at 25 °C, with degradation increasing with higher concentrations over 6 h[2]. D-Xylulose 5-phosphate sodium (5-100 mM GA3P or DHAP) is enzymatically synthesized from 5-100 mM GA3P or DHAP using cTK and TPI at 25 °C, with yield decreasing and initial production rate peaking at 80 mM as substrate concentration increases[2].
References:
[1]. Norman Hardt, et al. Facile synthesis of D-xylulose-5-phosphate and L-xylulose-5-phosphate by xylulokinase-catalyzed phosphorylation. 22 Jun 2019.
[2]. Shaeri J, et al. Characterization of enzymatic D-xylulose 5-phosphate synthesis. Biotechnol Bioeng. 2008;101(4):761-767.