Esterase (Synonyms: Ple;Ple ON Eupergit(R) C;Pig Liver Esterase, Immobilized ON Eupergit(R) C;Esterase;Esterase BS2;Esterase BS1;Esterase Solution Porcine Liver;Esterase SD) |
カタログ番号GC19922
One-Click Copy Product Info
|
豚肝臓からの固形物において、≥15単位/ mg
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 9016-18-6
Sample solution is provided at 25 µL, 10mM.
_1-3.$$$39978408@51.jpg');)
_1-9.$$$38538795@65.jpg');)
_1-9.$$$39112701@51.jpg');)
_1-7.$$$37316672@70.jpg');)
_366-372.$$$36198801@70.jpg');)
.$$$38565142@65.jpg');)
_1867-1883.$$$33248023@67.jpg');)
_eads6055.$$$39977546@45.jpg');)
_eadm9805.$$$40080571@45.jpg');)
_eadj3020.$$$39666821@45.jpg');)
_1-20.$$$39757301@28.jpg');)
_1-24.$$$38898113@28.jpg');)
_273-287.$$$36806353@46.jpg');)
_1-16.$$$37156877@44.jpg');)
_1-19.$$$37460805@44.jpg');)
_1291-1307.$$$34518654@46.jpg');)
Esterase, from pig liver, namely carboxylesterase, is responsible for the hydrolysis of carboxylesters into the corresponding alcohol and carboxylic acid. Esterase is principally expressed in tissues that maintain a barrier function, including the epithelia of the lung and gut, liver, kidney and skin[1]. Esterase is commonly used in vitro to verify whether esterases can hydrolyze or activate substrates, probes, or prodrugs. Esterase from porcine liver (0-25pg; 0-625amol) is used as a standard enzyme, with Fluorescein diacetate (FDA) as the fluorescent substrate, to establish a standard curve for esterase activity and to detect esterase activity in BY-2 tobacco cells and spruce embryogenic cells[2]. Esterase (10µL; 1.66U/µL) is used to pretreat the synthesized probe for 3.5h to induce de-esterification in advance and evaluate changes in background fluorescence[3].
References:
[1] Hatfield MJ, Umans RA, Hyatt JL, et al. Carboxylesterases: General detoxifying enzymes. Chem Biol Interact. 2016;259(Pt B):327-331.
[2] Vitecek J, Petrlova J, Adam V, Havel L, Kramer KJ, Babula P, Kizek R. A Fluorimetric Sensor for Detection of One Living Cell. Sensors. 2007; 7(3):222-238.
[3] Watkins RW, Lavis LD, Kung VM, Los GV, Raines RT. Fluorogenic affinity label for the facile, rapid imaging of proteins in live cells. Org Biomol Chem. 2009;7(19):3969-3975.
| Cas No. | 9016-18-6 | SDF | |
| 同義語 | Ple;Ple ON Eupergit(R) C;Pig Liver Esterase, Immobilized ON Eupergit(R) C;Esterase;Esterase BS2;Esterase BS1;Esterase Solution Porcine Liver;Esterase SD | ||
| Formula | N/A | M.Wt | N/A |
| 溶解度 | Storage | Store at -20℃ | |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Biological Activity: 28.6 u/mg solid Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 31 reference(s) in Google Scholar.)