SMCC-PE is a dipalmitoylphosphatidylethanolamine derivative modified with the heterobifunctional crosslinker SMCC. SMCC-PE is an intermediate formed by conjugating the SMCC linker with R-phycoerythrin for antibody conjugation [2].
In Vitro, SMCC-PE (egg PC/chol/SMCC-PE molar ratio 6:3:0.5; 2 h high vacuum drying, 14 min sonication, 20 h incubation at 4°C) forms part of stable, predominantly unilamellar 200 nm liposomes that enable covalent conjugation of SFP-3 peptide with a 10-20% coupling yield, producing a final peptide/total lipid ratio of 1:400[1]. SMCC (10 mg/mL; 1 h) reacts with purified PE at 25°C to form the activated SMCC-PE intermediate, with excess SMCC removed via size-exclusion chromatography[2].
References:
[1]. Puyal C, et al. Design of a short membrane-destabilizing peptide covalently bound to liposomes. Biochim Biophys Acta. 1994;1195(2):259-266.
[2]. Liang M, et al. In vitro evolution of diagnostic antibodies targeting native antigens in plasma by sandwich flow cytometry. Biotechnol J. 2024;19(2):e2300492.
In Vitro, SMCC-PE (egg PC/chol/SMCC-PE molar ratio 6:3:0.5; 2 h high vacuum drying, 14 min sonication, 20 h incubation at 4°C) forms part of stable, predominantly unilamellar 200 nm liposomes that enable covalent conjugation of SFP-3 peptide with a 10-20% coupling yield, producing a final peptide/total lipid ratio of 1:400[1]. SMCC (10 mg/mL; 1 h) reacts with purified PE at 25°C to form the activated SMCC-PE intermediate, with excess SMCC removed via size-exclusion chromatography[2].
References:
[1]. Puyal C, et al. Design of a short membrane-destabilizing peptide covalently bound to liposomes. Biochim Biophys Acta. 1994;1195(2):259-266.
[2]. Liang M, et al. In vitro evolution of diagnostic antibodies targeting native antigens in plasma by sandwich flow cytometry. Biotechnol J. 2024;19(2):e2300492.