SPDP-sulfo sodium is a water-soluble heterobifunctional crosslinker containing a cleavable disulfide bond. SPDP-sulfo sodium forms a stable amide bond with primary amines through the Sulfo-NHS ester at one end, and reacts with sulfhydryl groups via the 2-pyridyldithiol group at the other end, thereby constructing a disulfide crosslink that can be specifically cleaved in the intracellular reducing microenvironment. SPDP-sulfo sodium can be widely used in the research and construction of ADC s and immunotoxins, receptor-mediated nano-targeted carrier delivery systems, and enzyme-antibody/antigen labels [1] [2] [3] [4].
References:
[1]. Carlsson J, et al. Protein thiolation and reversible protein-protein conjugation. N-Succinimidyl 3-(2-pyridyldithio)propionate, a new heterobifunctional reagent. Biochem J. 1978;173(3):723-737.
[2]. Shukla R, et al. HER2 specific tumor targeting with dendrimer conjugated anti-HER2 mAb. Bioconjugate chemistry. 2006;17(5):1109-15.
[3]. Ghetie V, et al. Chemical construction of immunotoxins. Molecular biotechnology. 2001 Jul;18(3):251-68.
References:
[1]. Carlsson J, et al. Protein thiolation and reversible protein-protein conjugation. N-Succinimidyl 3-(2-pyridyldithio)propionate, a new heterobifunctional reagent. Biochem J. 1978;173(3):723-737.
[2]. Shukla R, et al. HER2 specific tumor targeting with dendrimer conjugated anti-HER2 mAb. Bioconjugate chemistry. 2006;17(5):1109-15.
[3]. Ghetie V, et al. Chemical construction of immunotoxins. Molecular biotechnology. 2001 Jul;18(3):251-68.