TSPBA is a bis-terminal phenylboronic acid-spacer diammonium salt crosslinker. TSPBA forms dynamic phenylboronate ester bonds with PVA, enabling the preparation of injectable self-healing hydrogels and endowing them with injectability, self-healing property, ROS-responsive degradation, tissue adhesion, and weak glucose-binding ability. TSPBA is applicable to research related to diabetic wounds [1].
In Vivo, TSPBA (3 w/v%; topical) -containing PATA hydrogel supports diabetic wound healing by scavenging ROS and exhibiting favorable biocompatibility with complete in vivo degradation by day 14, though optimal wound healing and primary blood glucose regulation require additional loaded agents in the PATA-GM (SF/LP) formulation[1].
In Vitro, TSPBA (3 w/v%; 2 days) containing PATA hydrogels disrupt MSSA, MRSA, and E. coli biofilms in vitro, as evidenced by SEM visualization of dispersed biofilms, with bacterial lysis restricted to SF-loaded hydrogel formulations[1]. TSPBA (3 w/v%; 2 h) containing PATA hydrogels scavenge over 60% of intracellular ROS in H2O2-stimulated L929 fibroblast cells in vitro[1]. TSPBA (3 w/v%; 24 h) containing PATA hydrogels do not reduce glucose concentrations in L929 fibroblast cell culture supernatants in vitro, while MH-loaded PATA hydrogel formulations do lower supernatant glucose levels[1].
References:
[1]. Chen R, et al. A multifunctional injectable, self-healing, and adhesive hydrogel-based wound dressing stimulated diabetic wound healing with combined reactive oxygen species scavenging, hyperglycemia reducing, and bacteria-killing abilities. Journal of nanobiotechnology. 2024 Jul 27;22(1):444.
In Vivo, TSPBA (3 w/v%; topical) -containing PATA hydrogel supports diabetic wound healing by scavenging ROS and exhibiting favorable biocompatibility with complete in vivo degradation by day 14, though optimal wound healing and primary blood glucose regulation require additional loaded agents in the PATA-GM (SF/LP) formulation[1].
In Vitro, TSPBA (3 w/v%; 2 days) containing PATA hydrogels disrupt MSSA, MRSA, and E. coli biofilms in vitro, as evidenced by SEM visualization of dispersed biofilms, with bacterial lysis restricted to SF-loaded hydrogel formulations[1]. TSPBA (3 w/v%; 2 h) containing PATA hydrogels scavenge over 60% of intracellular ROS in H2O2-stimulated L929 fibroblast cells in vitro[1]. TSPBA (3 w/v%; 24 h) containing PATA hydrogels do not reduce glucose concentrations in L929 fibroblast cell culture supernatants in vitro, while MH-loaded PATA hydrogel formulations do lower supernatant glucose levels[1].
References:
[1]. Chen R, et al. A multifunctional injectable, self-healing, and adhesive hydrogel-based wound dressing stimulated diabetic wound healing with combined reactive oxygen species scavenging, hyperglycemia reducing, and bacteria-killing abilities. Journal of nanobiotechnology. 2024 Jul 27;22(1):444.