Vitamin B12 |
カタログ番号GC18002
One-Click Copy Product Info
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ビタミンB12はビタミンです。
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 68-19-9
Sample solution is provided at 25 µL, 10mM.
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Vitamin B12 is a water-soluble vitamin containing cobalt, serving as a cofactor for methionine synthase and L-methylmalonyl-CoA mutase. Vitamin B12 can participate in methyl transfer reactions to promote erythrocyte development and maturation. Vitamin B12 helps maintain the integrity of the myelin sheath to reduce neurological damage. Vitamin B12 can be used in research related to megaloblastic anemia and neurological disorders[1-4].
In vitro, SH-SY5Y neuroblastoma cells were pretreated with Vitamin B12 (10nM) for 12h, followed by stimulation with H₂O₂ (50μM) for 24h. Vitamin B12 significantly increased phospholipid levels and protected plasmalogens from oxidative stress, while enhancing the expression of antioxidant enzymes[5]. HeLa, HL-60, and MCF-7 cancer cells were treated with Vitamin B12 (0.01–1000nM) in combination with 1,25(OH)₂D₃ for 24–72 hours. Vitamin B12 significantly enhanced the cytotoxicity of 1,25(OH)₂D₃ and increased the activation of caspase 4 and 8[6].
In vivo, Elmo1H/H Ins2Akita/+ diabetic mice were supplemented with Vitamin B12 (10mg/kg/day) via drinking water for 8 weeks. Vitamin B12 significantly reduced glomerular mesangial expansion and altered the expression of circadian clock genes[7]. In a myocardial ischemia/reperfusion injury model, C57BL/6 mice were administered Vitamin B12 (50mg/L; 4mL/day) via drinking water for 7 consecutive days prior to surgery. Vitamin B12 significantly alleviated myocardial ischemia/reperfusion injury[8].
References:
[1] Romain M, Sviri S, Linton DM, et al. The role of Vitamin B12 in the critically ill--a review. Anaesth Intensive Care. 2016 Jul;44(4):447-52.
[2] ZUCKNER J. Vitamin B12 therapy in osteoarthritis. Mo Med. 1954 Jun;51(6):450-1.
[3] Hasbaoui BE, Mebrouk N, Saghir S, et al. Vitamin B12 deficiency: case report and review of literature. Pan Afr Med J. 2021 Mar 4;38:237.
[4] Langan RC, Goodbred AJ. Vitamin B12 Deficiency: Recognition and Management. Am Fam Physician. 2017 Sep 15;96(6):384-389.
[5] Theiss EL, Griebsch LV, Lauer AA, et al. Vitamin B12 Attenuates Changes in Phospholipid Levels Related to Oxidative Stress in SH-SY5Y Cells. Cells. 2022 Aug 18;11(16):2574.
[6] Atoum MF, Alzoughool FE, Al-Mazaydeh ZA, et al. Tahtamouni LH. Vitamin B12 enhances the antitumor activity of 1,25-dihydroxyvitamin D3 via activation of caspases and targeting actin cytoskeleton. Tumour Biol. 2022;44(1):17-35.
[7] Wariyapperuma Appuhamillage NMW, Deshmukh AA, Moser RL, et al. Vitamin B12 Protects Against Early Diabetic Kidney Injury and Alters Clock Gene Expression in Mice. Biomolecules. 2025 Dec 3;15(12):1689.
[8] Qin Y, Shi Y, Yu Q, et al. Vitamin B12 alleviates myocardial ischemia/reperfusion injury via the SIRT3/AMPK signaling pathway. Biomed Pharmacother. 2023 Jul;163:114761.
| Cell experiment [1]: | |
Cell lines | SH-SY5Y neuroblastoma cells |
Preparation Method | SH-SY5Y cells were cultured in Dulbecco's Modified Eagle's Medium (DMEM) supplemented with 10% fetal bovine serum (FBS) at 37°C and 5% CO₂. When 80% confluence was reached, the FBS content was reduced to 1% for 16h, followed by incubation with 10nM Vitamin B12 for 24h. |
Reaction Conditions | Vitamin B12 (10nM); 12h; followed by stimulation with H₂O₂ (50μM); 24h |
Applications | Vitamin B12 treatment resulted in elevated levels of phosphatidylcholine (PC), phosphatidylethanolamine (PE), sphingomyelin (SM), and plasmalogens. Vitamin B12 protected plasmalogens from hydrogen peroxide (H₂O₂)-induced oxidative stress due to an elevated expression of the ROS-degrading enzymes superoxide dismutase (SOD) and catalase (CAT). Furthermore, Vitamin B12 elevates plasmalogen synthesis by increasing the expression of alkylglycerone phosphate synthase (AGPS) and choline phosphotransferase 1 (CHPT1) in SH-SY5Y cells exposed to H₂O₂-induced oxidative stress. |
| Animal experiment [2]: | |
Animal models | Elmo1H/H Ins2Akita/+ diabetic mice (C57BL/6J genetic background) |
Preparation Method | Mice were supplemented with Vitamin B12 (10mg/kg/day) via drinking water starting at eight weeks of age for eight weeks. |
Dosage form | 10mg/kg/day; drinking water; daily for 8 weeks |
Applications | Vitamin B12 treatment significantly reduced the percentage of glomeruli with mesangial expansion, improved systolic blood pressure and plasma triglyceride levels, and altered the expression of circadian rhythm genes in diabetic kidneys. |
References: | |
| Cas No. | 68-19-9 | SDF | |
| Canonical SMILES | CC1=CC2=C(C=C1C)N(C=N2)C3C(C(C(O3)CO)OP(=O)([O-])OC(C)CNC(=O)CCC4(C(C5C6(C(C(C(=C(C7=NC(=CC8=NC(=C(C4=N5)C)C(C8(C)C)CCC(=O)N)C(C7(C)CC(=O)N)CCC(=O)N)C)[N-]6)CCC(=O)N)(C)CC(=O)N)C)CC(=O)N)C)O.[C-]#N.[Co+3] | ||
| Formula | C63H88CoN14O14P | M.Wt | 1355.37 |
| 溶解度 | ≥ 45.65mg/mL in DMSO | Storage | Store at -20°C, protect from light |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 737.8 μL | 3.689 mL | 7.3781 mL |
| 5 mM | 147.6 μL | 737.8 μL | 1.4756 mL |
| 10 mM | 73.8 μL | 368.9 μL | 737.8 μL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 12 reference(s) in Google Scholar.)