ZnAF-1F tetraTFA is a potent fluorophore for with an Kd value of 2.2 nM. ZnAF-1F tetraTFA can be used as fluorescent probes for Zn2+ in cells. ZnAF-1F tetraTFA shows λ excitation of 489 nm and λ emission of 514 nm.
ZnAF-1F tetraTFA (1 μM) shows an Kon value of 3.5*106 M-1s-1, and an Koff value of 7.7*10-3 s-1 in 100 mM HEPES buffer[1].ZnAF-1F tetraTFA is a Zn sensors in neutral and slightly acidic conditions[2].
References:
[1]. Hirano T, et al. Improvement and biological applications of fluorescent probes for zinc, ZnAFs. J Am Chem Soc. 2002 Jun 12;124(23):6555-62.
[2]. Zhaohua Dai, et al. Tailoring tripodal ligands for zinc sensing. New J. Chem., 2007,31, 1708-1718. [3]. Que EL, et al. Metals in neurobiology: probing their chemistry and biology with molecular imaging. Chem Rev. 2008 May;108(5):1517-49.