Nuclease P1

Name: Nuclease P1
Brand: GlpBio
SKU: GF05226
Availability: InStock

Catalog No.: GF05226

Nuclease P1 is a single-stranded specific endonuclease, it hydrolyzes nucleic acids into 5'-mononucleotides and cleaves the single-stranded region of a double-stranded nucleic acid.

Los productos son exclusivamente para uso en investigación. No aptos para uso humano. No vendemos a pacientes.

Cas No.: 54576-84-0

250UIn Stock

$401.00

Pack Size	Price	Availability	Quantity	Action
250U	$401.00	In Stock	1

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Citas (Citations)

Actualmente no hay citas disponibles para este producto. A continuación se muestran publicaciones seleccionadas que citan productos GlpBio.

Nature
641, 529–536 (2025)
Nature
628, 630–638 (2024)
Nature
632, 686–694 (2024)
Nature
618, 1017–1023 (2023)
Nature
610, 366–372 (2022)
Cell
187(9):2288-2304 (2024)
Cell
183(7):1867-1883 (2020)
Science
388(6745) (2025)
Science
387(6739) (2025)
Science
387(6734) (2025)
Cell Research
35, 97–116 (2025)
Cell Research
34, 683–706 (2024)
Cell Research
33, 273–287 (2023)
Cell Research
33, 546–561 (2023)
Cell Research
33, 904–922 (2023)
Cell Research
31, 1291–1307 (2021)

Descripción (Description)

Nuclease P1 is a single-stranded specific endonuclease, it hydrolyzes nucleic acids into 5'-mononucleotides and cleaves the single-stranded region of a double-stranded nucleic acid. Nuclease P1 is one of the most well-known single stranded specific nucleases in the field of molecular biology, it is widely used in the pharmaceutical and food industries^[1]. Nuclease P1 can be obtained by fermentation of Penicillium citrinum: through extraction process, ultrafiltration concentration, drying and purification, etc.

References:
[1]. Mitiushkina NV, et al. Preparation of Duplex Sequencing Libraries for Archival Paraffin-Embedded Tissue Samples Using Single-Strand-Specific Nuclease P1. Int J Mol Sci. 2022 Apr 21;23(9):4586.

Protocolo (Protocol)

Reaction Conditions

pH Range: Effective pH Range: 4.0-8.0, Optimal pH Range: 4.5-5.5
Temperature Range: Effective Temperature Range: 35°C-85°C, Optimal Temperature Range: 65°C-75°C

Usage Method

Using yeast nucleic acid (RNA) as substrate: substrate concentration 2%-5%, recommended enzyme dosage 2%-4% (based on substrate dry matter), hydrolysis time 7-9 hours under optimal conditions;
Using yeast as substrate: yeast concentration 10%-12%, nuclease dosage 0.3%-0.5% (based on yeast dry matter), hydrolysis time 7-9 hours under optimal conditions.

Note: Due to differences in application areas and experimental parameters, the actual addition method and dosage of this product should be determined experimentally.

Documentos del producto (Product Documents)

Batch:Appearance:A solid

COA SDS Data Sheet

Propiedades químicas (Chemical Properties)

N.º CAS

54576-84-0

Solubilidad

H2O : 4.17 mg/mL (ultrasonic and warming and heat to 37°C)

Almacenamiento

Store at 4°C , do not freeze.

Consejos generales

Seleccione un disolvente adecuado según los datos de solubilidad del producto. Una vez preparada, divida la solución madre en alícuotas para evitar ciclos repetidos de congelación y descongelación.
Utilice las soluciones madre en un plazo de 6 meses si se conservan a -80°C, o de 1 mes si se conservan a -20°C.
Para mejorar la solubilidad, caliente el tubo a 37°C y soníquelo durante un periodo adecuado.

Condiciones de envío

Las muestras de evaluación se envían con acumuladores de frío. Los demás tamaños se envían a temperatura ambiente o, previa solicitud, con acumuladores de frío.

Calculadoras (Calculators)Molaridad / Dilución / Peso molecular / Conversión de unidades

Masa

Volumen

Concentración

Peso molecular

g/mol

Reviews

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