Endo H, Streptomyces picatus (Endo-β-N-acetylglucosaminidase H), isolated from Streptomyces plicatus, hydrolyzes the central glycosidic bond of the β1, 4-di-N-acetylchitobiose core in asparagine-linked oligosaccharides.
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| Pack Size | Price | Availability | Quantity | Action |
|---|---|---|---|---|
| 500U | $11.00 | In Stock | 1 | |
| 1KU | $18.00 | In Stock | 1 | |
| 5KU | $41.00 | In Stock | 1 |
Endo H, Streptomyces picatus (Endo-β-N-acetylglucosaminidase H), isolated from Streptomyces plicatus, hydrolyzes the central glycosidic bond of the β1, 4-di-N-acetylchitobiose core in asparagine-linked oligosaccharides[1].
References:
[1]. V Rao, et al. Crystal structure of endo-beta-N-acetylglucosaminidase H at 1.9 A resolution: active-site geometry and substrate recognition. Structure. 1995 May 15;3(5):449-57.
Instructions for Use:
1) Add 10 μg of RNase B to 1 μL of 500 mM sodium acetate (pH 7.5), and bring the volume to 10 μL with deionized water;
2) Add 1-2 μL of EndoH and gently mix by pipetting;
3) Incubate at 37°C for 1 h;
4) Analyze using SDS-PAGE or mass spectrometry.
Note: Incubation time or enzyme dosage needs to be optimized based on actual conditions.