Citas (Citations)
35 publicationsPublicaciones de investigadores que utilizan este productoDescripción (Description)
Phosphatase Inhibitor Cocktail I effectively inhibits alkaline phosphatases and serine/threonine protein phosphatases such as PP1 and PP2A.
Phosphorylation of proteins and lipids is a hallmark of the signaling or metabolic status of cells. A cocktail of three inhibitors that will inhibit alkaline phosphatases as well as serine/threonine protein phosphatases such as PP1 and PP2A. Each vial contains 1 ml phosphatase inhibitor cocktail solubilized in DMSO with the following components: Bromotetramisole oxalate, Cantharidin, and Microcystin-LR , Microcystis aeruginosa.
Phosphatase Inhibitor Cocktail I has been optimized and tested for mammalian cell and tissue extracts. This cocktail is supplied as a ready-to-use solution in DMSO.
Protocolo (Protocol)
Thaw at room temperature. Before assaying, add each solution at 1:100 (v/v) dilution to samples (such as cell lysates or tissue extracts). Mix the sample sufficiently after each time of addition.
Application Fields: Western Blot analysis, Co-IP, pull-down, IF, IHC, kinase assay and etc.
Documentos del producto (Product Documents)
Reviews
Review for Phosphatase Inhibitor Cocktail I (100× in DMSO)
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