(S)-Pro-xylane, a biologically active C-glycoside compound, is an important ingredient in antiaging skincare formulations in cosmetic products [1]. Through mimicking natural β-O-xylosides in skin, (S)-Pro-xylane is effective in stimulating the biosynthesis of glycosaminoglycans (GAGs), which leads to the preservation of matrix integrity and thereby contributes to skin's mechanical properties, such as elasticity and hydration [2]. (S)-Pro-xylane has been widely used to prevent the degradation and loss of glycosaminoglycans and proteoglycans in vivo[3].
In vitro, (S)-Pro-xylane treatment at 0.4mg/ml for 18h significantly enhanced the mRNA levels of collagen VII a1 and collagen IV a1 in normal human epidermal keratinocytes (NHKs), without affecting cell viability[4]. Treatment with 3mM (S)-Pro-xylane for 3 days increased syndecan 4 and perlecan levels in an ex vivo atrophic skin model[5].
In vivo, topical application of 100μl 1.5mg/ml (S)-Pro-xylane aqueous solution daily for 3 weeks significantly improved collagen loss and fiber breakage associated with skin aging, and reduced the degradation of elastic fibers associated with skin aging in Sprague-Dawley (SD) rats[6].
References:
[1] Zhao Y, Li Y, Shang J, et al. Engineering a Carbonyl Reductase for High-Efficiency Synthesis of Optically Pure (S)-Pro-Xylane: An Alternative Synthetic Route[J]. Journal of Agricultural and Food Chemistry, 2025, 73(16): 9759-9768.
[2] Dou Z, Hu P R, Song L L, et al. Extensive gene mining and facile engineering of a novel carbonyl reductase for asymmetric synthesis of anti-aging (S)-Pro-Xylane from d-xylose[J]. International Journal of Biological Macromolecules, 2025, 305: 140976.
[3] Lu J, Jiang Z, Xie X, et al. Efficient biosynthesis of Pro-Xylane through semi-rational engineering of carbonyl reductase from Canariomyces notabilis[J]. Molecular Catalysis, 2025, 579: 115020.
[4] Sok J, Pineau N, Dalko-Csiba M, et al. Improvement of the dermal epidermal junction in human reconstructed skin by a new c-xylopyranoside derivative[J]. European Journal of Dermatology, 2008, 18(3): 297-302.
[5] Pineau N, Bernerd F, Cavezza A, et al. A new C-xylopyranoside derivative induces skin expression of glycosaminoglycans and heparan sulphate proteoglycans[J]. European Journal of Dermatology, 2008, 18(1): 36-40.
[6] Yang A, Ma C, Song Q, et al. Anti-Aging Efficacy of Fructosazine and Deoxyfructosazine: A Comprehensive In Vitro and In Vivo Analysis[J]. Molecules, 2025, 30(11): 2263.