4-hydroxy Nonenal Glutathione-d3 (Synonyms: 4-HNE-GSH-d3) |
| Catalog No.GC92022 |
4-hydroxy Nonenal Glutathione-d3 (4-HNE-GSH-d3) is intended for use as an internal standard for the quantification of 4-HNE-GSH by GC- or LC-MS.
Products are for research use only. Not for human use. We do not sell to patients.
Sample solution is provided at 25 µL, 10mM.
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4-hydroxy Nonenal Glutathione-d3 (4-HNE-GSH-d3) is intended for use as an internal standard for the quantification of 4-HNE-GSH by GC- or LC-MS. 4-hydroxy-Nonenal (4-HNE) is a major aldehyde produced during the lipid peroxidation of ω-6 polyunsaturated fatty acids, such as arachidonic acid and linoleic acid.1,2 4-HNE-GSH is a major adduct formed by the reaction of 4-HNE with GSH.3,4,5,6 4-HNE-GSH levels in liver, plasma, or isolated cells can serve as biomarkers for oxidative stress.7 The trapping of 4-HNE by glutathione to give HNE-GSH prevents the formation of DNA adducts with 4-HNE.8,9 In human polymorphonuclear leukocytes, HNE-GSH is metabolized to 1,4-dihydroxynonene glutathione (DHN-GSH), 4-hydroxynonenoic acid glutathione (HNA-GSH), and 4-hydroxy nonenal mercapturic acid (HNE-MA).3
References:
[1]. Pryor, W.A., and Porter, N.A.Suggested mechanisms for the production of 4-hydroxy-2-nonenal from the autoxidation of polyunsaturated fatty acidsFree Radic. Biol. Med.8(6)541-543(1990).
[2]. Esterbauer, H., Schaur, R.J., and Zoliner, H.Chemistry and biochemistry of 4-hydroxynonenal, malonaldehyde, and related aldehydesFree Radic. Biol. Med.11(1)81-128(1991).
[3]. Siems, W., Crifo, C., Capuozzo, E., et al.Metabolism of 4-hydroxy-2-nonenal in human polymorphonuclear leukocytesArch. Biochem. Biophys.503(2)248-252(2010).
[4]. Laurent, A., Alary, J., Debrauwer, L., et al.Analysis in the rat of 4-hydroxynonenal metabolites excreted in bile: Evidence of enterohepatic circulation of these byproducts of lipid peroxidationChem. Res. Toxicol.12(10)887-894(1999).
[5]. Siems, W., and Grune, T.Intracellular metabolism of 4-hydroxynonenalMol. Aspects Med.24(4-5)167-175(2003).
[6]. Alary, J., Fernandez, Y., Debrauwer, L., et al.Identification of intermediate pathways of 4-hydroxynonenal metabolism in the ratChem. Res. Toxicol.16(3)320-327(2003).
[7]. V?lkel, W., Alvarez-Sánchez, R., Weick, I., et al.Glutathione conjugates of 4-hydroxy-2(E)-nonenal as biomarkers of hepatic oxidative stress-induced lipid peroxidation in ratsFree Radic. Biol. Med.38(11)1526-1536(2005).
[8]. King, A.O., Corley, E.G., Anderson, R.K., et al.An efficient synthesis of LTD4 antagonist L-699,392J. Org. Chem.58(14)3731-3735(1993).
[9]. Falletti, O., Cadet, J., Favier, A., et al.Trapping of 4-hydroxynonenal by glutathione efficiently prevents formation of DNA adducts in human cellsFree Radic. Biol. Med.42(8)1258-1269(2007).
| Cas No. | SDF | ||
| Synonyms | 4-HNE-GSH-d3 | ||
| Chemical Name | N5-((2R)-1-((carboxymethyl)amino)-3-(((3S)-5-hydroxy-2-(pentyl-5,5,5-d3)tetrahydrofuran-3-yl)thio)-1-oxopropan-2-yl)-D-glutamine | ||
| Canonical SMILES | O=C(NCC(O)=O)[C@H](CS[C@H]1CC(O)OC1CCCCC([2H])([2H])[2H])NC(CC[C@@H](N)C(O)=O)=O | ||
| Formula | C19H30D3N3O8S | M.Wt | 466.6 |
| Solubility | Water: 10 mg/ml | Storage | -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 2.1432 mL | 10.7158 mL | 21.4316 mL |
| 5 mM | 0.4286 mL | 2.1432 mL | 4.2863 mL |
| 10 mM | 0.2143 mL | 1.0716 mL | 2.1432 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
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Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >99.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)
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