2,5-Dioxopyrrolidin-1-yl 6-hydrazinylnicotinate hydrochloride is an intermediate of a radionuclide molecular probe targeting the CD4 receptor. 2,5-Dioxopyrrolidin-1-yl 6-hydrazinylnicotinate hydrochloride serves as an imaging agent for heart transplant rejection. This CD4 probe is prepared by mixing leukocyte differentiation antigen 4 monoclonal antibody with a solution of 6-hydrazinylnicotinoyl succinimide ester hydrochloride, followed by extraction and reaction to obtain 6-hydrazinylnicotinate-leukocyte differentiation antigen 4 monoclonal antibody; this antibody is then mixed with N-tris (hydroxymethyl) glycine, stannous chloride and technetium for an oscillatory reaction. This CD4 probe exhibits high affinity for CD4 + T lymphocytes, along with the characteristics of high sensitivity and high specificity, enabling early diagnosis of acute rejection [1].
References:
[1]. Xie Mingxing, et al. A preparation method of a radionuclide molecular probe targeting CD4 receptor and its application as an imaging agent for cardiac transplant rejection. 2020-01-15. CN111084889A.
References:
[1]. Xie Mingxing, et al. A preparation method of a radionuclide molecular probe targeting CD4 receptor and its application as an imaging agent for cardiac transplant rejection. 2020-01-15. CN111084889A.